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Dinamično uravnavanje (regulacija) metabolnih poti - Revision history
2024-03-29T13:37:20Z
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https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11129&oldid=prev
Katja Leben at 13:56, 5 January 2016
2016-01-05T13:56:56Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:56, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Farnezil pirofosfat (FPP) je pomembna izhodna spojina za mnoge organske spojin. Med drugim ga amorfo-4,11-dien sintaza (ADS) pretvori v amorfo-4,11-dien, ki je prekurzor artemizina, zdravila za malarijo. V visokih koncentracijah, ki jih dosežemo v sinteznobiološko pripravljenih celicah, je FPP toksičen in inhibira celično rast.<ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Farnezil pirofosfat (FPP) je pomembna izhodna spojina za mnoge organske spojin. Med drugim ga amorfo-4,11-dien sintaza (ADS) pretvori v amorfo-4,11-dien, ki je prekurzor artemizina, zdravila za malarijo. V visokih koncentracijah, ki jih dosežemo v sinteznobiološko pripravljenih celicah, je FPP toksičen in inhibira celično rast.<ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>S pomočjo računalniških orodij so raziskovalci zasnovali promotor, ki se odzove na stres in s tem posredno na FPP, ki je pri visokih koncentracijah povzročitelj stresnega okolja v celici. Pod nadzor tega promotorja so postavili set genov, ki sodelujejo v izoprenoidni metabolni poti in ločeno amorfo-4,11-dien sintetazo. Ob visoki koncentraciji FPP se stresni dejavniki vežejo na oba promotorja tako, da spodbudijo izražanje ADS in zavirajo izražanje proteinov, ki sodelujejo pri proizvodnji FPP. Dinamični sistem je bil več kot dvakrat bolj učinkovit od analognega statičnega sistema uravnavanja.<ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>S pomočjo računalniških orodij so raziskovalci zasnovali promotor, ki se odzove na stres in s tem posredno na FPP, ki je pri visokih koncentracijah povzročitelj stresnega okolja v celici. Pod nadzor tega promotorja so postavili set genov, ki sodelujejo v izoprenoidni metabolni poti in ločeno amorfo-4,11-dien sintetazo. Ob visoki koncentraciji FPP se stresni dejavniki vežejo na oba promotorja tako, da spodbudijo izražanje ADS in zavirajo izražanje proteinov, ki sodelujejo pri proizvodnji FPP. Dinamični sistem je bil več kot dvakrat bolj učinkovit od analognega statičnega sistema uravnavanja.<ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref><ref name="ref3">)Xu, P., Li, L., Zhang, F., Stephanopoulos, G. & Koffas, M. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc. Natl. Acad. Sci. U. S. A. 111, 11299–304 (2014)</ref></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">b</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><ref name="ref3">)Xu, P., Li, L., Zhang, F., Stephanopoulos, G. & Koffas, M. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc. Natl. Acad. Sci. U. S. A. 111, 11299–304 (2014)</ref></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td></tr>
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Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11128&oldid=prev
Katja Leben at 13:56, 5 January 2016
2016-01-05T13:56:29Z
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<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:56, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">a</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Farnezil pirofosfat (FPP) je pomembna izhodna spojina za mnoge organske spojin. Med drugim ga amorfo-4,11-dien sintaza (ADS) pretvori v amorfo-4,11-dien, ki je prekurzor artemizina, zdravila za malarijo. V visokih koncentracijah, ki jih dosežemo v sinteznobiološko pripravljenih celicah, je FPP toksičen in inhibira celično rast.<ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">S pomočjo računalniških orodij so raziskovalci zasnovali promotor, ki se odzove na stres in s tem posredno na FPP, ki je pri visokih koncentracijah povzročitelj stresnega okolja v celici. Pod nadzor tega promotorja so postavili set genov, ki sodelujejo v izoprenoidni metabolni poti in ločeno amorfo-4,11-dien sintetazo. Ob visoki koncentraciji FPP se stresni dejavniki vežejo na oba promotorja tako, da spodbudijo izražanje ADS in zavirajo izražanje proteinov, ki sodelujejo pri proizvodnji FPP. Dinamični sistem je bil več kot dvakrat bolj učinkovit od analognega statičnega sistema uravnavanja.</ins><ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>b</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>b</div></td></tr>
</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11127&oldid=prev
Katja Leben at 10:12, 5 January 2016
2016-01-05T10:12:44Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:12, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>b</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>b</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><ref name="<del style="font-weight: bold; text-decoration: none;">ref2</del>">)Xu, P., Li, L., Zhang, F., Stephanopoulos, G. & Koffas, M. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc. Natl. Acad. Sci. U. S. A. 111, 11299–304 (2014)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ref name="<ins style="font-weight: bold; text-decoration: none;">ref3</ins>">)Xu, P., Li, L., Zhang, F., Stephanopoulos, G. & Koffas, M. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc. Natl. Acad. Sci. U. S. A. 111, 11299–304 (2014)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Določeni statični regulatorji metabolnih poti so potencialno uporabni tudi kot dinamični regulatorji metabolnih poti. Eden takih je sistem s Cas9, ki ima okvarjeno nukleazno aktivnost (dCas9, nuclease deficient Cas9) in je zato sposobna le vezave DNA. Cas9 prepozna trinukleotidno zaporedje DNA (znano kot PAM) na podlagi kratkega segmenta CRISPR RNA (crRNA). <ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Določeni statični regulatorji metabolnih poti so potencialno uporabni tudi kot dinamični regulatorji metabolnih poti. Eden takih je sistem s Cas9, ki ima okvarjeno nukleazno aktivnost (dCas9, nuclease deficient Cas9) in je zato sposobna le vezave DNA. Cas9 prepozna trinukleotidno zaporedje DNA (znano kot PAM) na podlagi kratkega segmenta CRISPR RNA (crRNA). <ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Z načrtovanjem ustreznih crRNA lahko dosežemo vezavo dCas9 na poljubna zaporedja DNA, ki niso prisotna v naravi.<ref name="<del style="font-weight: bold; text-decoration: none;">ref2</del>">Esvelt, K. M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat. Methods 10, 1116–21 (2013)</ref> Zapis za dCas9 lahko vnesemo pod promotor, ki je pod vplivom naravnega LRTF. Tako bi preko odziva na naravni metabolit lahko vplivali tudi na sintezno metabolno pot, vendar takšni sistemi še niso bili eksperimentalno preizkušeni.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Z načrtovanjem ustreznih crRNA lahko dosežemo vezavo dCas9 na poljubna zaporedja DNA, ki niso prisotna v naravi.<ref name="<ins style="font-weight: bold; text-decoration: none;">ref4</ins>">Esvelt, K. M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat. Methods 10, 1116–21 (2013)</ref> Zapis za dCas9 lahko vnesemo pod promotor, ki je pod vplivom naravnega LRTF. Tako bi preko odziva na naravni metabolit lahko vplivali tudi na sintezno metabolno pot, vendar takšni sistemi še niso bili eksperimentalno preizkušeni.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references/></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references/></div></td></tr>
</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11126&oldid=prev
Katja Leben: /* Prva generacija dinamičnih kontrolnih sistemov */
2016-01-05T10:12:02Z
<p><span dir="auto"><span class="autocomment">Prva generacija dinamičnih kontrolnih sistemov</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:12, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">a</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><ref name="ref2">)Dahl, R. H. et al. Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039–46 (2013)</ref></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">b</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><ref name="ref2">)Xu, P., Li, L., Zhang, F., Stephanopoulos, G. & Koffas, M. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc. Natl. Acad. Sci. U. S. A. 111, 11299–304 (2014)</ref></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td></tr>
</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11123&oldid=prev
Katja Leben: /* Dinamični kontrolni sistemi */
2016-01-05T09:47:01Z
<p><span dir="auto"><span class="autocomment">Dinamični kontrolni sistemi</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:47, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Dinamični kontrolni sistemi so zelo koristni v primerih, kjer je določen metabolit toksičen za celico ali pa tekmuje z drugimi celičnimi komponentami pri pomembni celični funkciji. Potrebno je skrbno načrtovanje sistema z ozirom na hitrost produkcije metabolita in koncentracijo pri kateri se njegova škodljivost bistveno poveča.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Dinamični kontrolni sistemi so zelo koristni v primerih, kjer je določen metabolit toksičen za celico ali pa tekmuje z drugimi celičnimi komponentami pri pomembni celični funkciji. Potrebno je skrbno načrtovanje sistema z ozirom na hitrost produkcije metabolita in koncentracijo pri kateri se njegova škodljivost bistveno poveča.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Dvostopenjski sistemi uravnavanja (t. i. ON/OFF stikala) se ne upoštevajo kot dinamični sistem, saj ne vključujejo povratne regulacije in zahtevajo prisotnost induktorja.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><b>Dinamični kontrolni sistemi za uspešen nadzor metabolne poti zahtevajo:</b></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><b>Dinamični kontrolni sistemi za uspešen nadzor metabolne poti zahtevajo:</b></div></td></tr>
</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11122&oldid=prev
Katja Leben: /* Obeti na področju dinamičnega uravnavanja metabolnih poti */
2016-01-05T09:10:10Z
<p><span dir="auto"><span class="autocomment">Obeti na področju dinamičnega uravnavanja metabolnih poti</span></span></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:10, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Določeni statični regulatorji metabolnih poti so potencialno uporabni tudi kot dinamični regulatorji metabolnih poti. Eden takih je sistem s Cas9, ki ima okvarjeno nukleazno aktivnost (dCas9, nuclease deficient Cas9) in je zato sposobna le vezave DNA. Cas9 prepozna trinukleotidno zaporedje DNA (znano kot PAM) na podlagi kratkega segmenta CRISPR RNA (crRNA). <ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Določeni statični regulatorji metabolnih poti so potencialno uporabni tudi kot dinamični regulatorji metabolnih poti. Eden takih je sistem s Cas9, ki ima okvarjeno nukleazno aktivnost (dCas9, nuclease deficient Cas9) in je zato sposobna le vezave DNA. Cas9 prepozna trinukleotidno zaporedje DNA (znano kot PAM) na podlagi kratkega segmenta CRISPR RNA (crRNA). <ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Z načrtovanjem ustreznih crRNA lahko dosežemo vezavo dCas9 na poljubna zaporedja DNA, ki niso prisotna v naravi. <ref name="ref2">Esvelt, K. M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat. Methods 10, 1116–21 (2013)</ref>Zapis za dCas9 lahko vnesemo pod promotor, ki je pod vplivom naravnega LRTF. Tako bi preko odziva na naravni metabolit lahko vplivali tudi na sintezno metabolno pot, vendar takšni sistemi še niso bili eksperimentalno preizkušeni.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Z načrtovanjem ustreznih crRNA lahko dosežemo vezavo dCas9 na poljubna zaporedja DNA, ki niso prisotna v naravi.<ref name="ref2">Esvelt, K. M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat. Methods 10, 1116–21 (2013)</ref> Zapis za dCas9 lahko vnesemo pod promotor, ki je pod vplivom naravnega LRTF. Tako bi preko odziva na naravni metabolit lahko vplivali tudi na sintezno metabolno pot, vendar takšni sistemi še niso bili eksperimentalno preizkušeni.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td></tr>
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</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11121&oldid=prev
Katja Leben at 09:09, 5 January 2016
2016-01-05T09:09:46Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:09, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=== ''<del style="font-weight: bold; text-decoration: none;">Prvi val </del>dinamičnih kontrolnih sistemov'' ===</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>=== ''<ins style="font-weight: bold; text-decoration: none;">Prva generacija </ins>dinamičnih kontrolnih sistemov'' ===</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Tesno regulirana metabolna pot zahteva ustrezen pristop. Alosterična regulacija ali razgradnja encima sta učinkoviti naravni poti povratne regulacije, vendar trenutno razumevanje mehanizmov določanja specifičnosti še ni kos nalogi zasnovati sintezen specifičen alosterični regulator. Obstajajo pa naravni transkripcijski faktorji, ki se odzivajo na <del style="font-weight: bold; text-decoration: none;">ligand </del>(ligand – responsive transcription factor, LRTF) in jih s skrbnim načrtovanjem lahko uporabimo za povečanje izkoraistka naravnih metabolnih poti.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Tesno regulirana metabolna pot zahteva ustrezen pristop. Alosterična regulacija ali razgradnja encima sta učinkoviti naravni poti povratne regulacije, vendar trenutno razumevanje mehanizmov določanja specifičnosti še ni kos nalogi zasnovati sintezen specifičen alosterični regulator. Obstajajo pa naravni transkripcijski faktorji, ki se odzivajo na <ins style="font-weight: bold; text-decoration: none;">ligande </ins>(ligand – responsive transcription factor, LRTF) in jih s skrbnim načrtovanjem lahko uporabimo za povečanje izkoraistka naravnih metabolnih poti.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>LRTF vsebujejo domeno za vezavo metabolita (MBD) in DNA vezavno domeno (DBD). Ob vezavi metabolita pride do alosterične spremembe konformacije DNA vezavne domene. Glede na to, da ali ima v novi konformaciji LRTF večjo ali manjšo afiniteto do operatorske regije zavre ali sproži transkripcijo.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>LRTF vsebujejo domeno za vezavo metabolita (MBD) in DNA vezavno domeno (DBD). Ob vezavi metabolita pride do alosterične spremembe konformacije DNA vezavne domene. Glede na to, da ali ima v novi konformaciji LRTF večjo ali manjšo afiniteto do operatorske regije zavre ali sproži transkripcijo.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Pogosto LRTF vplivajo na več kot en gen – ko omogočijo transkripcijo enega gena tudi inhibirajo izražanje gena z nasprotnim učinkom. To nadvse zaželjeno lastnost so s pridom uporabili v nekaterih primerih uravnavanja metabolne poti, ki so predstavljeniv nadaljevanju.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Skupna točka vseh dinamično reguliranih poti so transkripcijski faktorji, ki se odzivajo na ligande (LTFR). Zankrat je njihova uporaba omejena na naravne LRFT, saj poznavanje mehanizmov alosteričnega prenosa informacije znotraj molekule še ni dovolj poznano, da bi z racionalnim dizajnom uspešno načrtovali specifične in funkcionalne sintezne LRTF.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=<del style="font-weight: bold; text-decoration: none;">= '''Obeti na področju dinamičnega uravnavanja </del>metabolnih poti<del style="font-weight: bold; text-decoration: none;">''' =</del>=</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Raziskovalci upajo, da bodo z uporabo računalniških orodij za prileganje odkrili regije odgovorne za prenos metabolit vezavno domeno in DNA vezavno odmeno. Do takrat pa bi lahko specifične transkripcijske faktorje za dinamičen sistem zasnovali v okviru naravnih LRTF. Po izbiri ustreznega LRTF bi morali pred sintezne TF vnesti ustrezne operatorje.<ref name</ins>=<ins style="font-weight: bold; text-decoration: none;">"ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Določeni statični regulatorji metabolnih poti so potencialno uporabni tudi kot dinamični regulatorji </ins>metabolnih poti<ins style="font-weight: bold; text-decoration: none;">. Eden takih je sistem s Cas9, ki ima okvarjeno nukleazno aktivnost (dCas9, nuclease deficient Cas9) in je zato sposobna le vezave DNA. Cas9 prepozna trinukleotidno zaporedje DNA (znano kot PAM) na podlagi kratkega segmenta CRISPR RNA (crRNA). <ref name</ins>=<ins style="font-weight: bold; text-decoration: none;">"ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Skupna točka vseh dinamično reguliranih poti so transkripcijski faktorji</del>, ki <del style="font-weight: bold; text-decoration: none;">se odzivajo na ligande </del>(<del style="font-weight: bold; text-decoration: none;">LTFR</del>). <del style="font-weight: bold; text-decoration: none;">Zankrat je njihova uporaba omejena </del>na <del style="font-weight: bold; text-decoration: none;">naravne V prihodnosti bodo raziskovalci </del>na <del style="font-weight: bold; text-decoration: none;">podlagi naravnih LRTF in informacij v bazah poskušali določiti regije odgovorne za prenos alosteričnega signala med metabolit in DNA vezavno domeno</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Z načrtovanjem ustreznih crRNA lahko dosežemo vezavo dCas9 na poljubna zaporedja DNA</ins>, ki <ins style="font-weight: bold; text-decoration: none;">niso prisotna v naravi. <ref name="ref2">Esvelt, K. M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat. Methods 10, 1116–21 </ins>(<ins style="font-weight: bold; text-decoration: none;">2013</ins>)<ins style="font-weight: bold; text-decoration: none;"></ref>Zapis za dCas9 lahko vnesemo pod promotor, ki je pod vplivom naravnega LRTF</ins>. <ins style="font-weight: bold; text-decoration: none;">Tako bi preko odziva </ins>na <ins style="font-weight: bold; text-decoration: none;">naravni metabolit lahko vplivali tudi </ins>na <ins style="font-weight: bold; text-decoration: none;">sintezno metabolno pot, vendar takšni sistemi še niso bili eksperimentalno preizkušeni.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol</ins>. <ins style="font-weight: bold; text-decoration: none;">36, 205–214 (2015)</ref></ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td></tr>
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</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11116&oldid=prev
Katja Leben: /* Obeti na področju dinamičnega uravnavanja metabolnih poti */
2016-01-05T08:04:10Z
<p><span dir="auto"><span class="autocomment">Obeti na področju dinamičnega uravnavanja metabolnih poti</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:04, 5 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Skupna točka vseh dinamično reguliranih poti so transkripcijski faktorji, ki se odzivajo na ligande (LTFR). Zankrat je njihova uporaba omejena na naravne V prihodnosti bodo raziskovalci na podlagi naravnih LRTF in informacij v bazah poskušali določiti regije odgovorne za prenos alosteričnega signala med <del style="font-weight: bold; text-decoration: none;">MBD </del>in <del style="font-weight: bold; text-decoration: none;">DBD</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Skupna točka vseh dinamično reguliranih poti so transkripcijski faktorji, ki se odzivajo na ligande (LTFR). Zankrat je njihova uporaba omejena na naravne V prihodnosti bodo raziskovalci na podlagi naravnih LRTF in informacij v bazah poskušali določiti regije odgovorne za prenos alosteričnega signala med <ins style="font-weight: bold; text-decoration: none;">metabolit </ins>in <ins style="font-weight: bold; text-decoration: none;">DNA vezavno domeno</ins>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references/></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><references/></div></td></tr>
</table>
Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11115&oldid=prev
Katja Leben at 23:59, 4 January 2016
2016-01-04T23:59:23Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 23:59, 4 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>== ''Prvi val dinamičnih kontrolnih sistemov'' ==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=</ins>== ''Prvi val dinamičnih kontrolnih sistemov'' <ins style="font-weight: bold; text-decoration: none;">=</ins>==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Tesno regulirana metabolna pot zahteva ustrezen pristop. Alosterična regulacija ali razgradnja encima sta učinkoviti naravni poti povratne regulacije, vendar trenutno razumevanje mehanizmov določanja specifičnosti še ni kos nalogi zasnovati sintezen specifičen alosterični regulator. Obstajajo pa naravni transkripcijski faktorji, ki se odzivajo na ligand (ligand – responsive transcription factor, LRTF) in jih s skrbnim načrtovanjem lahko uporabimo za povečanje izkoraistka naravnih metabolnih poti.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Tesno regulirana metabolna pot zahteva ustrezen pristop. Alosterična regulacija ali razgradnja encima sta učinkoviti naravni poti povratne regulacije, vendar trenutno razumevanje mehanizmov določanja specifičnosti še ni kos nalogi zasnovati sintezen specifičen alosterični regulator. Obstajajo pa naravni transkripcijski faktorji, ki se odzivajo na ligand (ligand – responsive transcription factor, LRTF) in jih s skrbnim načrtovanjem lahko uporabimo za povečanje izkoraistka naravnih metabolnih poti.<ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></div></td></tr>
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<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== '''Obeti na področju dinamičnega uravnavanja metabolnih poti''' ==</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Skupna točka vseh dinamično reguliranih poti so transkripcijski faktorji, ki se odzivajo na ligande (LTFR). Zankrat je njihova uporaba omejena na naravne V prihodnosti bodo raziskovalci na podlagi naravnih LRTF in informacij v bazah poskušali določiti regije odgovorne za prenos alosteričnega signala med MBD in DBD.</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''' Viri ''' ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
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Katja Leben
https://wiki.fkkt.uni-lj.si/index.php?title=Dinami%C4%8Dno_uravnavanje_(regulacija)_metabolnih_poti&diff=11114&oldid=prev
Katja Leben: /* Dinamični kontrolni sistemi */
2016-01-04T23:57:35Z
<p><span dir="auto"><span class="autocomment">Dinamični kontrolni sistemi</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 23:57, 4 January 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># biosenzor, ki zazna koncentracijo molekule ali okoljskega dejavnika, ki nas zanima</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># biosenzor, ki zazna koncentracijo molekule ali okoljskega dejavnika, ki nas zanima</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># prenos te informacije neposredno na aktuator (alosterična konformacijska sprememba ali sprememba interakcije med proteinoma – asociacija ali disociacija)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># prenos te informacije neposredno na aktuator (alosterična konformacijska sprememba ali sprememba interakcije med proteinoma – asociacija ali disociacija)</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)<del style="font-weight: bold; text-decoration: none;"><ref name="ref1">Cress, B. F., Trantas, E. A., Ververidis, F., Linhardt, R. J. & Koffas, M. A. Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways. Curr. Opin. Biotechnol. 36, 205–214 (2015)</ref></del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div># aktuator, pretvornik, ki sprejme signal in v odgovor prilagodi koncentracijo spojine, ki jo zaznavamo (njena razgradnja ali vpliv na izražanje gena)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''Prvi val dinamičnih kontrolnih sistemov'' ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== ''Prvi val dinamičnih kontrolnih sistemov'' ==</div></td></tr>
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Katja Leben