Metode za gojenje solinskih in morskih mikroorganizmov: Difference between revisions

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Gojišče MN
Gojišče MN
NaCl
[g/liter gojišča]
MgCI, . 6H20
NaNO3 -> 0.75
KC1
K2HPO4.3H2O -> 0.02
NaNO,
MgS04.7H2O -> 0.038
KaHPO4.3HzO
CaC12.2H20 -> 0.018
MgS04.7HzO
Citric acid -> 0.003
CaC1,. 2H20
Ferric ammonium citrate -> 0.003
Citric acid
EDTA -> 0.0005
Ferric ammonium citrate
Na2C03 -> 0.02
EDTA (disodium magnesium salt)
*Trace metal mix -> 1ml
Na2C03
Sea water -> 750 ml
Trace metal mix A5 + Co*
Deionized water -> 250 ml
Sea water
Deionized water


-
Gojišče ASN-I11
0.75
[g/liter gojišča]
0.02
NaCl -> 25.0
0.038
MgCI2.6H20 -> 2.0
0.018
KC1 -> 0.5
0.003
NaNO3 -> 0.75
0.003
K2HPO4.3H2O -> 0.02
0.0005
MgS04.7H2O -> 3.5
0.02
CaC12.2H20 -> 0.5
1 ml1-1
Citric acid -> 0.003
750 ml
Ferric ammonium citrate -> 0.003
250 ml
EDTA -> 0.0005
25.0
Na2C03 -> 0.02
Trace metal mix -> 1 ml
Deionized water -> 1000 ml


ASN-I11
*Trace metal mix:
2.0
[g/liter]
0.5
H3BO3 -> 2.86
0-75
MnCI2.4H20 -> 1.81
0.02
ZnSO4.7H2O -> 0.222
3.5
Na2MoO4.2H20 -> 0.390
0.5
CuSO4.5H20 -> 0.079
0.003
Co(N03)2.6H20 -> 0.00494
0.003
04005
0.02
1 ml1-1
1000 ml

Revision as of 14:14, 19 May 2018

Gojišča za cianobakterije: File:Gojisca.png [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]

Gojišče MN [g/liter gojišča] NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 0.038 CaC12.2H20 -> 0.018 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02

  • Trace metal mix -> 1ml

Sea water -> 750 ml Deionized water -> 250 ml

Gojišče ASN-I11 [g/liter gojišča] NaCl -> 25.0 MgCI2.6H20 -> 2.0 KC1 -> 0.5 NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 3.5 CaC12.2H20 -> 0.5 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02 Trace metal mix -> 1 ml Deionized water -> 1000 ml

  • Trace metal mix:

[g/liter] H3BO3 -> 2.86 MnCI2.4H20 -> 1.81 ZnSO4.7H2O -> 0.222 Na2MoO4.2H20 -> 0.390 CuSO4.5H20 -> 0.079 Co(N03)2.6H20 -> 0.00494