Metode za gojenje solinskih in morskih mikroorganizmov

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Gojišče MN
Gojišče MN
-
NaCl
+
[g/liter gojišča]
-
MgCI, . 6H20
+
NaNO3 -> 0.75
-
KC1
+
K2HPO4.3H2O -> 0.02
-
NaNO,
+
MgS04.7H2O -> 0.038
-
KaHPO4.3HzO
+
CaC12.2H20 -> 0.018
-
MgS04.7HzO
+
Citric acid -> 0.003
-
CaC1,. 2H20
+
Ferric ammonium citrate -> 0.003
-
Citric acid
+
EDTA -> 0.0005
-
Ferric ammonium citrate
+
Na2C03 -> 0.02
-
EDTA (disodium magnesium salt)
+
*Trace metal mix -> 1ml
-
Na2C03
+
Sea water -> 750 ml
-
Trace metal mix A5 + Co*
+
Deionized water -> 250 ml
-
Sea water
+
-
Deionized water
+
-
-
+
Gojišče ASN-I11
-
0.75
+
[g/liter gojišča]
-
0.02
+
NaCl -> 25.0
-
0.038
+
MgCI2.6H20 -> 2.0
-
0.018
+
KC1 -> 0.5
-
0.003
+
NaNO3 -> 0.75
-
0.003
+
K2HPO4.3H2O -> 0.02
-
0.0005
+
MgS04.7H2O -> 3.5
-
0.02
+
CaC12.2H20 -> 0.5
-
1 ml1-1
+
Citric acid -> 0.003
-
750 ml
+
Ferric ammonium citrate -> 0.003
-
250 ml
+
EDTA -> 0.0005
-
25.0
+
Na2C03 -> 0.02
 +
Trace metal mix -> 1 ml
 +
Deionized water -> 1000 ml
-
ASN-I11
+
*Trace metal mix:
-
2.0
+
[g/liter]
-
0.5
+
H3BO3 -> 2.86
-
0-75
+
MnCI2.4H20 -> 1.81
-
0.02
+
ZnSO4.7H2O -> 0.222
-
3.5
+
Na2MoO4.2H20 -> 0.390
-
0.5
+
CuSO4.5H20 -> 0.079
-
0.003
+
Co(N03)2.6H20 -> 0.00494
-
0.003
+
-
04005
+
-
0.02
+
-
1 ml1-1
+
-
1000 ml
+

Revision as of 14:14, 19 May 2018

Gojišča za cianobakterije: Image:Gojisca.png [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]

Gojišče MN [g/liter gojišča] NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 0.038 CaC12.2H20 -> 0.018 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02

  • Trace metal mix -> 1ml

Sea water -> 750 ml Deionized water -> 250 ml

Gojišče ASN-I11 [g/liter gojišča] NaCl -> 25.0 MgCI2.6H20 -> 2.0 KC1 -> 0.5 NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 3.5 CaC12.2H20 -> 0.5 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02 Trace metal mix -> 1 ml Deionized water -> 1000 ml

  • Trace metal mix:

[g/liter] H3BO3 -> 2.86 MnCI2.4H20 -> 1.81 ZnSO4.7H2O -> 0.222 Na2MoO4.2H20 -> 0.390 CuSO4.5H20 -> 0.079 Co(N03)2.6H20 -> 0.00494

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