Metode za gojenje solinskih in morskih mikroorganizmov

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Gojišča za cianobakterije:
Gojišča za cianobakterije:
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[[Image:gojisca.png]] [Rosmarie Rippka,  Josette Deruelles,  John B. Waterbury,  Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]
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Povzeto po članku: Rosmarie Rippka,  Josette Deruelles,  John B. Waterbury,  Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4
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Gojišče MN
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'''Gojišče MN'''
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[g/liter gojišča]
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''[g/liter gojišča]''
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NaNO3 -> 0.75
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*NaNO3 -> 0.75
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K2HPO4.3H2O -> 0.02
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*K2HPO4.3H2O -> 0.02
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MgS04.7H2O -> 0.038
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*MgS04.7H2O -> 0.038
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CaC12.2H20 -> 0.018
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*CaC12.2H20 -> 0.018
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Citric acid -> 0.003
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*Citric acid -> 0.003
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Ferric ammonium citrate -> 0.003
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*Ferric ammonium citrate -> 0.003
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EDTA -> 0.0005
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*EDTA -> 0.0005
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Na2C03 -> 0.02
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*Na2C03 -> 0.02
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*Trace metal mix -> 1ml
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*Trace metal mix -> 1ml '''(*)'''
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Sea water -> 750 ml
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*Sea water -> 750 ml
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Deionized water -> 250 ml
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*Deionized water -> 250 ml
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Gojišče ASN-I11
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'''Gojišče ASN-I11'''
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[g/liter gojišča]
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''[g/liter gojišča]''
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NaCl -> 25.0
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*NaCl -> 25.0
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MgCI2.6H20 -> 2.0
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*MgCI2.6H20 -> 2.0
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KC1 -> 0.5
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*KC1 -> 0.5
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NaNO3 -> 0.75
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*NaNO3 -> 0.75
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K2HPO4.3H2O -> 0.02
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*K2HPO4.3H2O -> 0.02
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MgS04.7H2O -> 3.5
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*MgS04.7H2O -> 3.5
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CaC12.2H20 -> 0.5
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*CaC12.2H20 -> 0.5
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Citric acid -> 0.003
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*Citric acid -> 0.003
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Ferric ammonium citrate -> 0.003
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*Ferric ammonium citrate -> 0.003
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EDTA -> 0.0005
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*EDTA -> 0.0005
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Na2C03 -> 0.02
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*Na2C03 -> 0.02
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Trace metal mix -> 1 ml
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*Trace metal mix -> 1 ml '''(*)'''
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Deionized water -> 1000 ml
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*Deionized water -> 1000 ml
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*Trace metal mix:
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'''(*) Trace metal mix:'''
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[g/liter]
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''[g/liter]''
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H3BO3 -> 2.86
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*H3BO3 -> 2.86
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MnCI2.4H20 -> 1.81
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*MnCI2.4H20 -> 1.81
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ZnSO4.7H2O -> 0.222
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*ZnSO4.7H2O -> 0.222
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Na2MoO4.2H20 -> 0.390
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*Na2MoO4.2H20 -> 0.390
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CuSO4.5H20 -> 0.079
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*CuSO4.5H20 -> 0.079
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Co(N03)2.6H20 -> 0.00494
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*Co(N03)2.6H20 -> 0.00494

Current revision

Gojišča za cianobakterije: Povzeto po članku: Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4

Gojišče MN [g/liter gojišča]

  • NaNO3 -> 0.75
  • K2HPO4.3H2O -> 0.02
  • MgS04.7H2O -> 0.038
  • CaC12.2H20 -> 0.018
  • Citric acid -> 0.003
  • Ferric ammonium citrate -> 0.003
  • EDTA -> 0.0005
  • Na2C03 -> 0.02
  • Trace metal mix -> 1ml (*)
  • Sea water -> 750 ml
  • Deionized water -> 250 ml

Gojišče ASN-I11 [g/liter gojišča]

  • NaCl -> 25.0
  • MgCI2.6H20 -> 2.0
  • KC1 -> 0.5
  • NaNO3 -> 0.75
  • K2HPO4.3H2O -> 0.02
  • MgS04.7H2O -> 3.5
  • CaC12.2H20 -> 0.5
  • Citric acid -> 0.003
  • Ferric ammonium citrate -> 0.003
  • EDTA -> 0.0005
  • Na2C03 -> 0.02
  • Trace metal mix -> 1 ml (*)
  • Deionized water -> 1000 ml

(*) Trace metal mix: [g/liter]

  • H3BO3 -> 2.86
  • MnCI2.4H20 -> 1.81
  • ZnSO4.7H2O -> 0.222
  • Na2MoO4.2H20 -> 0.390
  • CuSO4.5H20 -> 0.079
  • Co(N03)2.6H20 -> 0.00494
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