Metode za gojenje solinskih in morskih mikroorganizmov

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Gojišča za cianobakterije: Image:Gojisca.png [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]

Gojišče MN [g/liter gojišča] NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 0.038 CaC12.2H20 -> 0.018 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02

  • Trace metal mix -> 1ml

Sea water -> 750 ml Deionized water -> 250 ml

Gojišče ASN-I11 [g/liter gojišča] NaCl -> 25.0 MgCI2.6H20 -> 2.0 KC1 -> 0.5 NaNO3 -> 0.75 K2HPO4.3H2O -> 0.02 MgS04.7H2O -> 3.5 CaC12.2H20 -> 0.5 Citric acid -> 0.003 Ferric ammonium citrate -> 0.003 EDTA -> 0.0005 Na2C03 -> 0.02 Trace metal mix -> 1 ml Deionized water -> 1000 ml

  • Trace metal mix:

[g/liter] H3BO3 -> 2.86 MnCI2.4H20 -> 1.81 ZnSO4.7H2O -> 0.222 Na2MoO4.2H20 -> 0.390 CuSO4.5H20 -> 0.079 Co(N03)2.6H20 -> 0.00494

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