https://wiki.fkkt.uni-lj.si/index.php?title=RNaza_H&feed=atom&action=history
RNaza H - Revision history
2024-03-28T11:02:20Z
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Tisa Ževart at 23:14, 23 January 2014
2014-01-23T23:14:35Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>RNaza H je encim, ki <del style="font-weight: bold; text-decoration: none;">ga najdemo skoraj </del>v <del style="font-weight: bold; text-decoration: none;">vseh </del>organizmih<del style="font-weight: bold; text-decoration: none;">, tako pri arhejah </del>in <del style="font-weight: bold; text-decoration: none;">prokariontih kot </del>pri <del style="font-weight: bold; text-decoration: none;">evkariontih</del>. Je standardni <del style="font-weight: bold; text-decoration: none;">[[</del>encim<del style="font-weight: bold; text-decoration: none;">]] </del>v tehnologiji rDNA.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Ribonukleaza H (</ins>RNaza H<ins style="font-weight: bold; text-decoration: none;">) </ins>je <ins style="font-weight: bold; text-decoration: none;">endonukleaza, torej </ins>encim, ki <ins style="font-weight: bold; text-decoration: none;">katalizira hidrolizo notranjih fosfoestrskih vezi v RNA. <sup>[[RNazaH#Viri|[1]]]</sup>. Najdemo jo lahko </ins>v <ins style="font-weight: bold; text-decoration: none;">evkariontskih in prokariontskih </ins>organizmih<ins style="font-weight: bold; text-decoration: none;">. RNaza H prepozna dupleks DNA-RNA </ins>in <ins style="font-weight: bold; text-decoration: none;">cepi RNA na naključnih mestih. Uporabljamo jo za odstranjevanje mRNA </ins>pri <ins style="font-weight: bold; text-decoration: none;">sintezi cDNA</ins>. Je standardni encim v tehnologiji rDNA.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>RNaza H je bila prvič identificirana v telečjem timusu. Najbolje raziskan član družine Rnaze H pa je RNaza HI, ki jo najdemo v E.coli.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>RNaza H je bila prvič identificirana v telečjem timusu. Najbolje raziskan član družine Rnaze H pa je RNaza HI, ki jo najdemo v E.coli.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">RNaza </del>H <del style="font-weight: bold; text-decoration: none;">katalizira razcep RNA. Spada med hidrolaze (E</del>.C.<del style="font-weight: bold; text-decoration: none;">: 3.1.26.4),</del><<del style="font-weight: bold; text-decoration: none;">ref</del>><del style="font-weight: bold; text-decoration: none;">http://www.chem.qmul.ac.uk/iubmb/enzyme/</del></<del style="font-weight: bold; text-decoration: none;">ref</del>> <del style="font-weight: bold; text-decoration: none;"> torej katalizira razcep vezi s hidrolizo</del>. <del style="font-weight: bold; text-decoration: none;">RNaza H prepozna dupleks DNA-RNA in cepi RNA na naključnih mestih. Aktivna </del>je <del style="font-weight: bold; text-decoration: none;">pri reverznih transkriptazah</del>, <del style="font-weight: bold; text-decoration: none;">kar pomeni da katalizira sintezo cDNA na osnovi matrice mRNA. Pogost kofaktor, ki je pri uporabljen pri hidrolizi je magnezijev ion</del>.<ref>http://<del style="font-weight: bold; text-decoration: none;">en</del>.<del style="font-weight: bold; text-decoration: none;">wikipedia</del>.<del style="font-weight: bold; text-decoration: none;">org</del>/<del style="font-weight: bold; text-decoration: none;">wiki</del>/<del style="font-weight: bold; text-decoration: none;">RNase_H</del></ref> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">3-D struktura RNaze </ins>H <ins style="font-weight: bold; text-decoration: none;">je običajno sestavljena iz β-strukture, ki jo obdajajo α-vijačnice</ins>. <ins style="font-weight: bold; text-decoration: none;">Lahko pa, tako kot pri RNazi H, najdeni v virusu HIV-1, manjka ena od α-vijačnic, </ins>C<ins style="font-weight: bold; text-decoration: none;">-vijačnica, ki poveča sposobnost vezave</ins>. <<ins style="font-weight: bold; text-decoration: none;">sup</ins>><ins style="font-weight: bold; text-decoration: none;">[[RNazaH#Viri|[2]]]</ins></<ins style="font-weight: bold; text-decoration: none;">sup</ins>>. <ins style="font-weight: bold; text-decoration: none;">Njena molekulska masa </ins>je <ins style="font-weight: bold; text-decoration: none;">18</ins>,<ins style="font-weight: bold; text-decoration: none;">5 kDa</ins>.<ref>http://<ins style="font-weight: bold; text-decoration: none;">www</ins>.<ins style="font-weight: bold; text-decoration: none;">neb</ins>.<ins style="font-weight: bold; text-decoration: none;">com</ins>/<ins style="font-weight: bold; text-decoration: none;">nebecomm</ins>/<ins style="font-weight: bold; text-decoration: none;">products/faqproductM0297.asp</ins></ref> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Sestavljena </del>je <del style="font-weight: bold; text-decoration: none;">iz petih β</del>-<del style="font-weight: bold; text-decoration: none;">verig</del>, <del style="font-weight: bold; text-decoration: none;">ki so obdane z α-vijačnicami</del>. <del style="font-weight: bold; text-decoration: none;">Njena molekulska masa je 18</del>,<del style="font-weight: bold; text-decoration: none;">5 kDa</del>.<<del style="font-weight: bold; text-decoration: none;">ref</del>><del style="font-weight: bold; text-decoration: none;">http://www.neb.com/nebecomm/products/faqproductM0297.asp</del></<del style="font-weight: bold; text-decoration: none;">ref</del>> <del style="font-weight: bold; text-decoration: none;"> </del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">RNaza H ima pomembno vlogo pri dozorevanju virusa HIV in </ins>je <ins style="font-weight: bold; text-decoration: none;">ena glavnih tarč farmacevtskih raziskav pri iskanju zdravila proti AIDSu. Pri procesu sinteze DNA v virusu je RNaza H del encima reverzna transkriptaza in degradira RNA v DNA</ins>-<ins style="font-weight: bold; text-decoration: none;">RNA hibridu. S tem osvobodi enoverižno DNA</ins>, <ins style="font-weight: bold; text-decoration: none;">komplementarno R sekvenci na 3' koncu mRNA retrotranspozona</ins>. <ins style="font-weight: bold; text-decoration: none;">Osvobojeni del verige DNA se premesti na 3' konec mRNA retrotranspozona</ins>, <ins style="font-weight: bold; text-decoration: none;">pri čemer se sinteza komplementarne verige DNA nadaljuje</ins>. <<ins style="font-weight: bold; text-decoration: none;">sup</ins>><ins style="font-weight: bold; text-decoration: none;">[[RNazaH#Viri|[3]]]</ins></<ins style="font-weight: bold; text-decoration: none;">sup</ins>><ins style="font-weight: bold; text-decoration: none;">. </ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">RNaza H je kot del reverzne transkriptaze nujno potrebna za širjenje retrovirusov kot sta npr. HIV in virus mišje levkemije. Zaviralci tega encima bi torej lahko zagotovili zdravila proti boleznim kot je aids, zato je raziskovanje RNaze H zelo pomembno v farmaciji in medicini.<ref>http://en.wikipedia.org/wiki/RNase_H</ref> </del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">VIRI</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Viri ==</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* <sup>[1]</sup> [http://books.google.si/books/about/Temelji_biokemije.html?id=c8CpAAAACAAJ&redir_esc=y R. Boyer: Temelji biokemije, stran 261-263]</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">----</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* <sup>[2]</sup> [http://en.wikipedia.org/wiki/RNase_H RNase H, Wikipedia] </ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* <sup>[2]</sup> [http://bib.irb.hr/datoteka/97768.Diplomski.pdf L. Ester: Aktivnost retrotranspozona Tpv2 u stanicama uročnjaka Arabidopsis thaliana L., stran 6]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://www.chem.qmul.ac.uk/iubmb/enzyme/</ref></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://www.chem.qmul.ac.uk/iubmb/enzyme/</ref></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://en.wikipedia.org/wiki/RNase_H</ref> </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://en.wikipedia.org/wiki/RNase_H</ref> </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://www.neb.com/nebecomm/products/faqproductM0297.asp</ref> </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><ref>http://www.neb.com/nebecomm/products/faqproductM0297.asp</ref> </div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Zunanje povezave ==</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">*[http://en.wikipedia.org/wiki/RNase_H RNase H, Wikipedia]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"><ref></del>[[Category:LEX]]<del style="font-weight: bold; text-decoration: none;"></ref></del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Category:LEX]]</div></td></tr>
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Tisa Ževart
https://wiki.fkkt.uni-lj.si/index.php?title=RNaza_H&diff=7714&oldid=prev
Danijela Hodnik: New page: RNaza H ---- RNaza H je encim, ki ga najdemo skoraj v vseh organizmih, tako pri arhejah in prokariontih kot pri evkariontih. Je standardni encim v tehnologiji rDNA. RNaza H je bila ...
2013-01-06T18:46:42Z
<p>New page: RNaza H ---- RNaza H je encim, ki ga najdemo skoraj v vseh organizmih, tako pri arhejah in prokariontih kot pri evkariontih. Je standardni <a href="/index.php?title=Encim&action=edit&redlink=1" class="new" title="Encim (page does not exist)">encim</a> v tehnologiji rDNA. RNaza H je bila ...</p>
<p><b>New page</b></p><div>RNaza H<br />
<br />
----<br />
<br />
RNaza H je encim, ki ga najdemo skoraj v vseh organizmih, tako pri arhejah in prokariontih kot pri evkariontih. Je standardni [[encim]] v tehnologiji rDNA.<br />
<br />
RNaza H je bila prvič identificirana v telečjem timusu. Najbolje raziskan član družine Rnaze H pa je RNaza HI, ki jo najdemo v E.coli.<br />
<br />
RNaza H katalizira razcep RNA. Spada med hidrolaze (E.C.: 3.1.26.4),<ref>http://www.chem.qmul.ac.uk/iubmb/enzyme/</ref> torej katalizira razcep vezi s hidrolizo. RNaza H prepozna dupleks DNA-RNA in cepi RNA na naključnih mestih. Aktivna je pri reverznih transkriptazah, kar pomeni da katalizira sintezo cDNA na osnovi matrice mRNA. Pogost kofaktor, ki je pri uporabljen pri hidrolizi je magnezijev ion.<ref>http://en.wikipedia.org/wiki/RNase_H</ref> <br />
<br />
Sestavljena je iz petih β-verig, ki so obdane z α-vijačnicami. Njena molekulska masa je 18,5 kDa.<ref>http://www.neb.com/nebecomm/products/faqproductM0297.asp</ref> <br />
<br />
RNaza H je kot del reverzne transkriptaze nujno potrebna za širjenje retrovirusov kot sta npr. HIV in virus mišje levkemije. Zaviralci tega encima bi torej lahko zagotovili zdravila proti boleznim kot je aids, zato je raziskovanje RNaze H zelo pomembno v farmaciji in medicini.<ref>http://en.wikipedia.org/wiki/RNase_H</ref> <br />
<br />
VIRI<br />
<br />
----<br />
<ref>http://www.chem.qmul.ac.uk/iubmb/enzyme/</ref><br />
<ref>http://en.wikipedia.org/wiki/RNase_H</ref> <br />
<ref>http://www.neb.com/nebecomm/products/faqproductM0297.asp</ref> <br />
<br />
<ref>[[Category:LEX]]</ref></div>
Danijela Hodnik