Reverzna transkriptaza: Difference between revisions
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Odkrila sta jo neodvisno drug od drugega Howard Temin, University of Wisconsin-Madison in David Baltimore, MIT (Massachusetts Institute of Technology). Skupaj z Renatom Dulbeccom so za ta dosežek leta 1975 prejeli Nobelovo nagrado. | Odkrila sta jo neodvisno drug od drugega Howard Temin, University of Wisconsin-Madison in David Baltimore, MIT (Massachusetts Institute of Technology). Skupaj z Renatom Dulbeccom so za ta dosežek leta 1975 prejeli Nobelovo nagrado. | ||
Katalizira procese v: | Katalizira procese v: <sup>[[Reverzna transkriptaza#Viri|[2]]]</sup> | ||
* retrovirusih | * retrovirusih | ||
* hepadnavirusih in caulimovirusih | * hepadnavirusih in caulimovirusih | ||
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== Viri == | == Viri == | ||
* <sup>[1]</sup> [http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html] | * <sup>[1]</sup> [http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html] | ||
* <sup>[2]</sup> [http://pfam.sanger.ac.uk/family?acc=PF00078 http://pfam.sanger.ac.uk/family?acc=PF00078] | |||
Revision as of 13:55, 12 December 2010
Reverzna transkriptaza je encim (EC 2.7.7.49), ki spada v razred transferaz. Katalizira podaljševanje DNA verige na 3'- koncu na osnovi RNA matrice, torej omogoča prepis RNA na DNA (en. RNA-directed DNA polymerase). Za začetek potrebuje že obstoječ RNA ali DNA primer – ne more ustvariti verige od začetka ("de novo" ).[1] Prepis poteka tako, da na obstoječi primer dodaja posamezne nukleotide, ki so komplementarni RNA matrici, torej katalizira proces, ki poteka v nasprotni smeri kakor transkripcija
Odkrila sta jo neodvisno drug od drugega Howard Temin, University of Wisconsin-Madison in David Baltimore, MIT (Massachusetts Institute of Technology). Skupaj z Renatom Dulbeccom so za ta dosežek leta 1975 prejeli Nobelovo nagrado.
Katalizira procese v: [2]
- retrovirusih
- hepadnavirusih in caulimovirusih
- retrotransposonih
- bakterijki msDNA
- intronih tipa II
Reakcija, ki jo katalizira: deoxynucleoside triphosphate + <math>DNA_n</math> = diphosphate + <math>DNA_{n+1}</math>
