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https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14294
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-19T14:17:41Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
Povzeto po članku: Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4<br />
<br />
'''Gojišče MN'''<br />
''[g/liter gojišča]''<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 0.038<br />
*CaC12.2H20 -> 0.018<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1ml '''(*)'''<br />
*Sea water -> 750 ml<br />
*Deionized water -> 250 ml<br />
<br />
'''Gojišče ASN-I11'''<br />
''[g/liter gojišča]''<br />
*NaCl -> 25.0<br />
*MgCI2.6H20 -> 2.0<br />
*KC1 -> 0.5<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 3.5<br />
*CaC12.2H20 -> 0.5<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1 ml '''(*)'''<br />
*Deionized water -> 1000 ml<br />
<br />
'''(*) Trace metal mix:'''<br />
''[g/liter]''<br />
*H3BO3 -> 2.86<br />
*MnCI2.4H20 -> 1.81<br />
*ZnSO4.7H2O -> 0.222<br />
*Na2MoO4.2H20 -> 0.390<br />
*CuSO4.5H20 -> 0.079<br />
*Co(N03)2.6H20 -> 0.00494</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14293
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-19T14:17:00Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
Povzeto po članku: Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4<br />
<br />
Gojišče MN<br />
[g/liter gojišča]<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 0.038<br />
*CaC12.2H20 -> 0.018<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1ml (*)<br />
*Sea water -> 750 ml<br />
*Deionized water -> 250 ml<br />
<br />
Gojišče ASN-I11<br />
[g/liter gojišča]<br />
*NaCl -> 25.0<br />
*MgCI2.6H20 -> 2.0<br />
*KC1 -> 0.5<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 3.5<br />
*CaC12.2H20 -> 0.5<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1 ml (*)<br />
*Deionized water -> 1000 ml<br />
<br />
(*) Trace metal mix:<br />
[g/liter]<br />
*H3BO3 -> 2.86<br />
*MnCI2.4H20 -> 1.81<br />
*ZnSO4.7H2O -> 0.222<br />
*Na2MoO4.2H20 -> 0.390<br />
*CuSO4.5H20 -> 0.079<br />
*Co(N03)2.6H20 -> 0.00494</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14292
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-19T14:16:28Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]<br />
<br />
Gojišče MN<br />
[g/liter gojišča]<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 0.038<br />
*CaC12.2H20 -> 0.018<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1ml (*)<br />
*Sea water -> 750 ml<br />
*Deionized water -> 250 ml<br />
<br />
Gojišče ASN-I11<br />
[g/liter gojišča]<br />
*NaCl -> 25.0<br />
*MgCI2.6H20 -> 2.0<br />
*KC1 -> 0.5<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 3.5<br />
*CaC12.2H20 -> 0.5<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1 ml (*)<br />
*Deionized water -> 1000 ml<br />
<br />
(*) Trace metal mix:<br />
[g/liter]<br />
*H3BO3 -> 2.86<br />
*MnCI2.4H20 -> 1.81<br />
*ZnSO4.7H2O -> 0.222<br />
*Na2MoO4.2H20 -> 0.390<br />
*CuSO4.5H20 -> 0.079<br />
*Co(N03)2.6H20 -> 0.00494</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14291
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-19T14:15:28Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]<br />
<br />
Gojišče MN<br />
[g/liter gojišča]<br />
*NaNO3 -> 0.75<br />
*K2HPO4.3H2O -> 0.02<br />
*MgS04.7H2O -> 0.038<br />
*CaC12.2H20 -> 0.018<br />
*Citric acid -> 0.003<br />
*Ferric ammonium citrate -> 0.003<br />
*EDTA -> 0.0005<br />
*Na2C03 -> 0.02<br />
*Trace metal mix -> 1ml<br />
*Sea water -> 750 ml<br />
*Deionized water -> 250 ml<br />
<br />
Gojišče ASN-I11<br />
[g/liter gojišča]<br />
NaCl -> 25.0<br />
MgCI2.6H20 -> 2.0<br />
KC1 -> 0.5<br />
NaNO3 -> 0.75<br />
K2HPO4.3H2O -> 0.02<br />
MgS04.7H2O -> 3.5<br />
CaC12.2H20 -> 0.5<br />
Citric acid -> 0.003<br />
Ferric ammonium citrate -> 0.003<br />
EDTA -> 0.0005<br />
Na2C03 -> 0.02<br />
Trace metal mix -> 1 ml<br />
Deionized water -> 1000 ml<br />
<br />
*Trace metal mix:<br />
[g/liter]<br />
H3BO3 -> 2.86<br />
MnCI2.4H20 -> 1.81<br />
ZnSO4.7H2O -> 0.222<br />
Na2MoO4.2H20 -> 0.390<br />
CuSO4.5H20 -> 0.079<br />
Co(N03)2.6H20 -> 0.00494</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14290
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-19T14:14:46Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]<br />
<br />
Gojišče MN<br />
[g/liter gojišča]<br />
NaNO3 -> 0.75<br />
K2HPO4.3H2O -> 0.02<br />
MgS04.7H2O -> 0.038<br />
CaC12.2H20 -> 0.018<br />
Citric acid -> 0.003<br />
Ferric ammonium citrate -> 0.003<br />
EDTA -> 0.0005<br />
Na2C03 -> 0.02<br />
*Trace metal mix -> 1ml<br />
Sea water -> 750 ml<br />
Deionized water -> 250 ml<br />
<br />
Gojišče ASN-I11<br />
[g/liter gojišča]<br />
NaCl -> 25.0<br />
MgCI2.6H20 -> 2.0<br />
KC1 -> 0.5<br />
NaNO3 -> 0.75<br />
K2HPO4.3H2O -> 0.02<br />
MgS04.7H2O -> 3.5<br />
CaC12.2H20 -> 0.5<br />
Citric acid -> 0.003<br />
Ferric ammonium citrate -> 0.003<br />
EDTA -> 0.0005<br />
Na2C03 -> 0.02<br />
Trace metal mix -> 1 ml<br />
Deionized water -> 1000 ml<br />
<br />
*Trace metal mix:<br />
[g/liter]<br />
H3BO3 -> 2.86<br />
MnCI2.4H20 -> 1.81<br />
ZnSO4.7H2O -> 0.222<br />
Na2MoO4.2H20 -> 0.390<br />
CuSO4.5H20 -> 0.079<br />
Co(N03)2.6H20 -> 0.00494</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14289
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-18T18:39:27Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]<br />
<br />
Gojišče MN<br />
NaCl<br />
MgCI, . 6H20<br />
KC1<br />
NaNO,<br />
KaHPO4.3HzO<br />
MgS04.7HzO<br />
CaC1,. 2H20<br />
Citric acid<br />
Ferric ammonium citrate<br />
EDTA (disodium magnesium salt)<br />
Na2C03<br />
Trace metal mix A5 + Co*<br />
Sea water<br />
Deionized water<br />
<br />
-<br />
0.75<br />
0.02<br />
0.038<br />
0.018<br />
0.003<br />
0.003<br />
0.0005<br />
0.02<br />
1 ml1-1<br />
750 ml<br />
250 ml<br />
25.0<br />
<br />
ASN-I11<br />
2.0<br />
0.5<br />
0-75<br />
0.02<br />
3.5<br />
0.5<br />
0.003<br />
0.003<br />
04005<br />
0.02<br />
1 ml1-1<br />
1000 ml</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14288
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-18T18:37:07Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] ([Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 1-61])</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14287
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-18T18:35:26Z
<p>Jakob Mraz: </p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]] (Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 1-61)</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Metode_za_gojenje_solinskih_in_morskih_mikroorganizmov&diff=14286
Metode za gojenje solinskih in morskih mikroorganizmov
2018-05-18T18:33:37Z
<p>Jakob Mraz: New page: Gojišča za cianobakterije: Image:gojisca.png</p>
<hr />
<div>Gojišča za cianobakterije:<br />
[[Image:gojisca.png]]</div>
Jakob Mraz
https://wiki.fkkt.uni-lj.si/index.php?title=Soline18_-_metode&diff=14285
Soline18 - metode
2018-05-18T18:30:31Z
<p>Jakob Mraz: </p>
<hr />
<div>Seznam metod, uporabljenih v okviru študentskega projekta Soline18.<br />
<br />
* [[Metode za gojenje solinskih in morskih mikroorganizmov]]<br />
* Metode za izolacijo mikroorganizmov<br />
* [[Metode za fiksacijo in makroskopsko analizo petole]]<br />
* Metode za izolacijo DNA iz solinskega blata<br />
* Metode za analizo obstojnosti DNA v okoljskih vzorcih<br />
* Metode za določanje pestrosti mikrobiote<br />
* Metode za določanje slanosti<br />
* Metode za določanje protimikrobnega delovanja</div>
Jakob Mraz