https://wiki.fkkt.uni-lj.si/api.php?action=feedcontributions&feedformat=atom&user=Nives+Ra%C5%BEnjevi%C4%87 2026-04-15T11:58:06Z User contributions MediaWiki 1.39.3 https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15886 2019-05-23T14:39:32Z

<p>Nives Ražnjević: /* Optimisation procedure */</p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX edited by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimisation of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with the inserted sequence for GFP driven by MND promoter. GFP expression was measured by flow cytometry. Meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;<br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage of the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15885 2019-05-23T14:37:03Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) [[Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah]]. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) [[Optimizacija večvalentnega peptidnega cepiva za nikotinsko odvisnost]]. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) [[Načrtovanje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen]]. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). [http://wiki.fkkt.uni-lj.si/index.php/Metabolno_in%C5%BEenirstvo_bakterije_Escherichia_coli_za_de_novo_sintezo_vitamina_B12 Metabolno inženirstvo bakterije ''Escherichia coli'' za ''de novo'' sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;]. Valentina Novak<br /> # Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in ''Streptomyces coelicolor'' (Z. Xu ''et al'', Appl. Environ. Microbiol. 85(7), 2019, https://doi.org/10.1128/AEM.03005-18). [http://wiki.fkkt.uni-lj.si/index.php/Mutageneza_celotnega_genoma_odkriva_povezave_med_metabolnimi_potmi_in_produkcijo_aktinorhodina_v_Streptomyces_coelicolor Mutageneza celotnega genoma odkriva povezave med metabolnimi potmi in produkcijo aktinorhodina v ''Streptomyces coelicolor''.] David Titovšek<br /> # Cost-effective production of recombinant peptides in ''Escherichia coli'' (A. Gaglione ''et al'', N. Biotechnol. 51, 2019, https://doi.org/10.1016/j.nbt.2019.02.004.) [http://wiki.fkkt.uni-lj.si/index.php/Stro%C5%A1kovno_u%C4%8Dinkovita_proizvodnja_rekombinantnih_peptidov_v_Escherichia_coli Stroškovno učinkovita proizvodnja rekombinantnih peptidov v ''Escherichia coli''] Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Metabolic engineering of the thermophilic filamentous fungus ''Myceliophthora thermophila'' to produce fumaric acid (S. Gu et al.; Biotechnology for Biofuels, 11(1), 2019; https://doi.org/10.1186/s13068-018-1319-1)[http://wiki.fkkt.uni-lj.si/index.php/Metabolni_inženiring_termofilne_filamentozne_glive_Myceliophthora_thermophila_za_proizvodnjo_fumarne_kisline Metabolni inženiring termofilne filamentozne glive ''Myceliophthora thermophila'' za proizvodnjo fumarne kisline]Primož Bembič<br /> # Karin Dobravc Škof<br /> <br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Potential of sustainable bioenergy production from ''Synechocystis'' sp. cultivated in wastewater at large scale – A low cost biorefinery approach (V. Ashokkumar, W.-H. Shen, C. Ngamcharussrivichai, E. Agila in F. N. Ani; Energy Conversion and Management 186, 2019; https://doi.org/10.1016/j.enconman.2019.02.056) [http://wiki.fkkt.uni-lj.si/index.php/Potencial_trajnostne_proizvodnje_bioenergije_iz_Synechocystis_sp.%2C_gojene_v_odpadnih_vodah_v_velikem_merilu_%E2%80%93_pristop_nizkocenovne_biorafinerije Potencial trajnostne proizvodnje bioenergije iz ''Synechocystis'' sp., gojene v odpadnih vodah v velikem merilu – pristop nizkocenovne biorafinerije] Maksimiljan Adamek<br /> # Life-cycle assessment of biofuel production from microalgae via various bioenergy conversion systems (C.-H. Sun ''et al.''; Energy 171, 2019; https://doi.org/10.1016/j.energy.2019.01.074) [http://wiki.fkkt.uni-lj.si/index.php/Napoved_%C5%BEivljenjskega_cikla_proizvodnje_biogoriva_iz_mikroalg_preko_razli%C4%8Dnih_sistemov_pretvorbe_bioenergije Napoved življenjskega cikla proizvodnje biogoriva iz mikroalg preko različnih sistemov pretvorbe bioenergije] Aljoša Marinko<br /> # ''n''-Butanol and ethanol production from cellulose by ''Clostridium cellulovorans'' overexpressing heterologous aldehyde/alcohol dehydrogenases (T. Bao, J. Zhao, J. Li, X. Liu in S.-T. Yang; Bioresource Technology 285, 2019; https://doi.org/10.1016/j.biortech.2019.121316) [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_n-butanola_in_etanola_iz_celuloze_v_bakteriji_Clostridium_cellulovorans_s_prekomernim_izra%C5%BEanjem_aldehid_in_alkohol_dehidrogenaz Proizvodnja ''n''-butanola in etanola iz celuloze v bakteriji ''Clostridium cellulovorans'' s prekomernim izražanjem aldehid in alkohol dehidrogenaz] Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development (Shi L, Luo X, Jiang J, Hu T, et al. National Science Review, nwz043, https://doi.org/10.1093/nsr/nwz043)[http://wiki.fkkt.uni-lj.si/index.php/V_transgenskih_opicah_rhesus%2C_ki_so_prena%C5%A1alke_vstavljenega_%C4%8Dlove%C5%A1kega_gena_MCPH1%2C_je_opazen_podoben_razvoj_mo%C5%BEganov%2C_kot_pri_%C4%8Dloveku V transgenskih opicah rhesus, ki so prenašalke vstavljenega človeškega gena MCPH1, je opazen podoben razvoj možganov, kot pri človeku] Katja Kunčič<br /> # Dynamic DNA material with emergent locomotion behavior powered by artificial metabolism (Hamada in sod., Sci. Robotics, 2019; https://doi.org/10.1126/scirobotics.aaw3512) [http://wiki.fkkt.uni-lj.si/index.php/Dinami%C4%8Den_DNA_nanomaterial_s_porajajo%C4%8Dim_se_obna%C5%A1anjem_lokomocije Dinamičen DNA nanomaterial s porajajočim se obnašanjem lokomocije] Peter Pečan<br /> # Role of contacts in long-range protein conductance (B. Zhang et al.; PNAS 119 (13), 2019) https://www.pnas.org/content/116/13/5886) [http://wiki.fkkt.uni-lj.si/index.php/Vloga_stikov_pri_proteinski_prevodnosti_na_velike_razdalnje Vloga stikov pri proteinski prevodnosti na velike razdalnje] Jaka Kos<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012.&lt;/noinclude&gt;). [http://wiki.fkkt.uni-lj.si/index.php/In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije Inženirstvo spreminjanja plazmatk s popravljanjem primarnih človeških celic B na osnovi homologije]. Nives Ražnjević<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15884 2019-05-23T14:29:22Z

<p>Nives Ražnjević: /* Conclusion */</p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX edited by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with the inserted sequence for GFP driven by MND promoter. GFP expression was measured by flow cytometry. Meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;<br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage of the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15883 2019-05-23T14:26:58Z

<p>Nives Ražnjević: /* Optimisation procedure */</p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX edited by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with the inserted sequence for GFP driven by MND promoter. GFP expression was measured by flow cytometry. Meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;<br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15882 2019-05-23T14:26:05Z

<p>Nives Ražnjević: /* Optimisation procedure */</p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX edited by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with the inserted sequence for GFP driven by MND promoter. GFP expression was measured by flow cytometry, meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;<br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15881 2019-05-23T14:23:58Z

<p>Nives Ražnjević: </p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX edited by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with inserted GFP sequence driven by MND promoter. GFP expression was measured by flow cytometry, meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt; <br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15880 2019-05-23T14:22:00Z

<p>Nives Ražnjević: </p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with inserted GFP sequence driven by MND promoter. GFP expression was measured by flow cytometry, meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt; <br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15879 2019-05-23T14:20:58Z

<p>Nives Ražnjević: </p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce ''de novo'' protein, it opens up an opportunity for the production of deficient protein (in the terms of the disease) and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with inserted GFP sequence driven by MND promoter. GFP expression was measured by flow cytometry, meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt; <br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15878 2019-05-23T14:19:05Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) [[Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah]]. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) [[Optimizacija večvalentnega peptidnega cepiva za nikotinsko odvisnost]]. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) [[Načrtovanje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen]]. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). [http://wiki.fkkt.uni-lj.si/index.php/Metabolno_in%C5%BEenirstvo_bakterije_Escherichia_coli_za_de_novo_sintezo_vitamina_B12 Metabolno inženirstvo bakterije ''Escherichia coli'' za ''de novo'' sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;]. Valentina Novak<br /> # Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in ''Streptomyces coelicolor'' (Z. Xu ''et al'', Appl. Environ. Microbiol. 85(7), 2019, https://doi.org/10.1128/AEM.03005-18). [http://wiki.fkkt.uni-lj.si/index.php/Mutageneza_celotnega_genoma_odkriva_povezave_med_metabolnimi_potmi_in_produkcijo_aktinorhodina_v_Streptomyces_coelicolor Mutageneza celotnega genoma odkriva povezave med metabolnimi potmi in produkcijo aktinorhodina v ''Streptomyces coelicolor''.] David Titovšek<br /> # Cost-effective production of recombinant peptides in ''Escherichia coli'' (A. Gaglione ''et al'', N. Biotechnol. 51, 2019, https://doi.org/10.1016/j.nbt.2019.02.004.) [http://wiki.fkkt.uni-lj.si/index.php/Stro%C5%A1kovno_u%C4%8Dinkovita_proizvodnja_rekombinantnih_peptidov_v_Escherichia_coli Stroškovno učinkovita proizvodnja rekombinantnih peptidov v ''Escherichia coli''] Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Metabolic engineering of the thermophilic filamentous fungus ''Myceliophthora thermophila'' to produce fumaric acid (S. Gu et al.; Biotechnology for Biofuels, 11(1), 2019; https://doi.org/10.1186/s13068-018-1319-1)[http://wiki.fkkt.uni-lj.si/index.php/Metabolni_inženiring_termofilne_filamentozne_glive_Myceliophthora_thermophila_za_proizvodnjo_fumarne_kisline Metabolni inženiring termofilne filamentozne glive ''Myceliophthora thermophila'' za proizvodnjo fumarne kisline]Primož Bembič<br /> # Karin Dobravc Škof<br /> <br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Potential of sustainable bioenergy production from ''Synechocystis'' sp. cultivated in wastewater at large scale – A low cost biorefinery approach (V. Ashokkumar, W.-H. Shen, C. Ngamcharussrivichai, E. Agila in F. N. Ani; Energy Conversion and Management 186, 2019; https://doi.org/10.1016/j.enconman.2019.02.056) [http://wiki.fkkt.uni-lj.si/index.php/Potencial_trajnostne_proizvodnje_bioenergije_iz_Synechocystis_sp.%2C_gojene_v_odpadnih_vodah_v_velikem_merilu_%E2%80%93_pristop_nizkocenovne_biorafinerije Potencial trajnostne proizvodnje bioenergije iz ''Synechocystis'' sp., gojene v odpadnih vodah v velikem merilu – pristop nizkocenovne biorafinerije] Maksimiljan Adamek<br /> # Life-cycle assessment of biofuel production from microalgae via various bioenergy conversion systems (C.-H. Sun ''et al.''; Energy 171, 2019; https://doi.org/10.1016/j.energy.2019.01.074) [http://wiki.fkkt.uni-lj.si/index.php/Napoved_%C5%BEivljenjskega_cikla_proizvodnje_biogoriva_iz_mikroalg_preko_razli%C4%8Dnih_sistemov_pretvorbe_bioenergije Napoved življenjskega cikla proizvodnje biogoriva iz mikroalg preko različnih sistemov pretvorbe bioenergije] Aljoša Marinko<br /> # ''n''-Butanol and ethanol production from cellulose by ''Clostridium cellulovorans'' overexpressing heterologous aldehyde/alcohol dehydrogenases (T. Bao, J. Zhao, J. Li, X. Liu in S.-T. Yang; Bioresource Technology 285, 2019; https://doi.org/10.1016/j.biortech.2019.121316) [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_n-butanola_in_etanola_iz_celuloze_v_bakteriji_Clostridium_cellulovorans_s_prekomernim_izra%C5%BEanjem_aldehid_in_alkohol_dehidrogenaz Proizvodnja ''n''-butanola in etanola iz celuloze v bakteriji ''Clostridium cellulovorans'' s prekomernim izražanjem aldehid in alkohol dehidrogenaz] Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development (Shi L, Luo X, Jiang J, Hu T, et al. National Science Review, nwz043, https://doi.org/10.1093/nsr/nwz043)[http://wiki.fkkt.uni-lj.si/index.php/V_transgenskih_opicah_rhesus%2C_ki_so_prena%C5%A1alke_vstavljenega_%C4%8Dlove%C5%A1kega_gena_MCPH1%2C_je_opazen_podoben_razvoj_mo%C5%BEganov%2C_kot_pri_%C4%8Dloveku V transgenskih opicah rhesus, ki so prenašalke vstavljenega človeškega gena MCPH1, je opazen podoben razvoj možganov, kot pri človeku] Katja Kunčič<br /> # Dynamic DNA material with emergent locomotion behavior powered by artificial metabolism (Hamada in sod., Sci. Robotics, 2019; https://doi.org/10.1126/scirobotics.aaw3512) [http://wiki.fkkt.uni-lj.si/index.php/Dinami%C4%8Den_DNA_nanomaterial_s_porajajo%C4%8Dim_se_obna%C5%A1anjem_lokomocije Dinamičen DNA nanomaterial s porajajočim se obnašanjem lokomocije] Peter Pečan<br /> # Role of contacts in long-range protein conductance (B. Zhang et al.; PNAS 119 (13), 2019) https://www.pnas.org/content/116/13/5886) [http://wiki.fkkt.uni-lj.si/index.php/Vloga_stikov_pri_proteinski_prevodnosti_na_velike_razdalnje Vloga stikov pri proteinski prevodnosti na velike razdalnje] Jaka Kos<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012&lt;/noinclude&gt;). [http://wiki.fkkt.uni-lj.si/index.php/In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije Inženirstvo spreminjanja plazmatk s popravljanjem primarnih človeških celic B na osnovi homologije]. Nives Ražnjević<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=In%C5%BEenirstvo_spreminjanja_plazmatk_s_popravljanjem_primarnih_%C4%8Dlove%C5%A1kih_celic_B_na_osnovi_homologije&diff=15877 2019-05-23T14:17:43Z

<p>Nives Ražnjević: New page: [https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) w...</p> <hr /> <div>[https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Hemophilia-B Haemophilia B] is a disease caused by a deficiency in the secretion of coagulation factor IX (FIX) which results in the impaired blood coagulation cascade&lt;ref name=&quot;ena&quot;&gt;Nathwani ''et al''. (2011). Adenovirusassociated virus vector-mediated gene transfer in hemophilia B. N. ''Engl. J. Med.'' 365, 2357–2365&lt;/ref&gt;. Thus, the disease is manifested by blood dotting defects. Considering the plasma cells’ ability to produce de novo protein, it opens up an opportunity for the production of deficient protein (in the terms of the disease) and thus the potential cure for protein-deficiency diseases. This review article will focus on the methods used to investigate the ability of activated B cells differentiated from primary naive human B cells to produce functioning factor IX by [https://www.addgene.org/crispr/guide/CRISPR/Cas9 CRISPR/Cas9] genome editing tool and [https://blog.addgene.org/crispr-101-homology-directed-repair homology-directed repair (HDR)] &lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Optimisation procedure=<br /> Prior to testing efficacy of B cells genome editing, respective optimisation of the experimental conditions had to be made. The latter has consisted of four crucial steps, namely: <br /> # '''Optimization of cell expansion conditions''' – by the cultivation of B cells with the “B cell activation cocktail” approximately 36-fold expansion was achieved<br /> # '''Screening the successfulness of construct insertion''' – two specific genome regions were selected for editing with RNP complexes containing respective guidance RNAs. Site-specific insertion was confirmed by Illumina sequencing.<br /> # '''Screening the influence of genome editing region on the primary naïve human B cell differentiation''' – altogether, 5 genomic regions were selected: four of them are important for B cell differentiation (''IRF4, PRDM1, PAX5, BACH2''). The fifth region, ''CCR5'', has no significant influence on B cell differentiation. By disruption of the listed regions, their function was confirmed and thus appropriate region for insertion of factor IX sequence could be selected. Cell differentiation level was measured via flow cytometry. <br /> # '''Selection of adequate template donor vector''' – scAAV was designed with inserted GFP sequence driven by MND promoter. GFP expression was measured by flow cytometry, meanwhile, minimal loss of cell viability was observed. B cells transduced with AAV serotype 6 showed the highest mean fluorescence.&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt; <br /> <br /> =Testing the efficacy of genome editing=<br /> The genome of primary human B cells was edited in terms of insertion of two genes – one for factor IX (whose production was the main goal of the research) and the other for BAFF (B cell activating factor)&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of factor IX==<br /> The region used for genome editing was ''CCR5'' region of the B cells’ genome for which it was shown (in prior experimental conditions testing) that it has no significant effect on B cell differentiation if edited. Two parallel experiments were conducted, the one containing only Cas9 RNP with gRNA for the respective region and AAV with donor template for insertion of FIX and the other, where under the same conditions of FIX insertion, ''PAX5'' region was inhibited to induce a higher rate of B cell differentiation. However, no significant difference in differentiation was shown. <br /> The insertion of factor IX nucleotide sequence was successful, as well as the expression of the respective protein in comparison to control. The presence of produced factor IX was determined by ELISA method. The activity of produced factor IX was tested afterwards with chromogenic assay and high-specific-activity has been shown in the vitamin K supplemented culture&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> ==Insertion and expression of BAFF==<br /> For BAFF it is known that it provides activation and survival of B cells. The idea is that naïve primary B cells would be doubly edited, which means that while expressing functioning factor IX, it would at the same time produce enough BAFF for its own sustainment by autocrine secretion. The expression of BAFF was determined by ELISA method&lt;ref name=&quot;dve&quot;&gt;Hung ''et al''. (2018). Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells. ''Mol. Ther.'' 26(2), 456-467.&lt;/ref&gt;. <br /> <br /> =Conclusion=<br /> In this research, conducted by Hung ''et al''., B cells were successfully used as a “cell factory” for production of active factor IX. Since the method of editing of B cells’ genome was CRISPR/Cas9 editing, the significance of the research lies in the opening opportunity for further usage the respective method to edit B cells’ genome for production of some other protein whose deficiency is causing anomalies. Also, optimisation conditions for the successful experiment were determined which sets up the appropriate ground for further research in this field such as studying mutations which may cause autoimmune diseases.<br /> <br /> =References=<br /> &lt;references/&gt;</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15761 2019-05-06T15:07:07Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) [[Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah]]. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) [[Optimizacija večvalentnega peptidnega cepiva za nikotinsko odvisnost]]. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) [[Načrtovanje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen]]. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). [http://wiki.fkkt.uni-lj.si/index.php/Metabolno_in%C5%BEenirstvo_bakterije_Escherichia_coli_za_de_novo_sintezo_vitamina_B12 Metabolno inženirstvo bakterije ''Escherichia coli'' za ''de novo'' sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;]. Valentina Novak<br /> # Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in ''Streptomyces coelicolor'' (Z. Xu ''et al'', Appl. Environ. Microbiol. 85(7), 2019, https://doi.org/10.1128/AEM.03005-18). [http://wiki.fkkt.uni-lj.si/index.php/Mutageneza_celotnega_genoma_odkriva_povezave_med_metabolnimi_potmi_in_produkcijo_aktinorhodina_v_Streptomyces_coelicolor Mutageneza celotnega genoma odkriva povezave med metabolnimi potmi in produkcijo aktinorhodina v ''Streptomyces coelicolor''.] David Titovšek<br /> # Cost-effective production of recombinant peptides in ''Escherichia coli'' (A. Gaglione ''et al'', N. Biotechnol. 51, 2019, https://doi.org/10.1016/j.nbt.2019.02.004.) [http://wiki.fkkt.uni-lj.si/index.php/Stro%C5%A1kovno_u%C4%8Dinkovita_proizvodnja_rekombinantnih_peptidov_v_Escherichia_coli Stroškovno učinkovita proizvodnja rekombinantnih peptidov v ''Escherichia coli''] Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> <br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # ''n''-Butanol and ethanol production from cellulose by ''Clostridium cellulovorans'' overexpressing heterologous aldehyde/alcohol dehydrogenases (T. Bao, J. Zhao, J. Li, X. Liu in S.-T. Yang; Bioresource Technology 285, 2019; https://doi.org/10.1016/j.biortech.2019.121316) Proizvodnja ''n''-butanola in etanola iz celuloze v bakteriji ''Clostridium cellulovorans'' s prekomernim izražanjem aldehid in alkohol dehidrogenaz. Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Katja Kunčič<br /> # Peter Pečan<br /> # Jaka Kos<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012&lt;/noinclude&gt;). Inženirstvo spreminjanja plazmatk s popravljanjem primarnih človeških celic B na osnovi homologije. Nives Ražnjević<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15653 2019-04-16T19:34:06Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) [[Optimizacija večvalentnega peptidnega cepiva za nikotinsko odvisnost]]. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovanje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012&lt;/noinclude&gt;). Inženirstvo spreminjanja plazmatk s popravljanjem primarnih človeških celic B na osnovi homologije. Nives Ražnjević<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15513 2019-04-13T14:49:15Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012&lt;/noinclude&gt;). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15512 2019-04-13T14:48:40Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. &lt;noinclude&gt;https://doi.org/10.1016/j.ymthe.2017.11.012&lt;/noinclude&gt;). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15509 2019-04-13T11:17:21Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: 10.1016/j.ymthe.2017.11.012). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15508 2019-04-13T11:16:57Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: [[[http:// 10.1016/j.ymthe.2017.11.012]]]). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15507 2019-04-13T11:15:19Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: [http:// 10.1016/j.ymthe.2017.11.012]). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15506 2019-04-13T11:14:20Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: [10.1016/j.ymthe.2017.11.012]). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15505 2019-04-13T11:12:56Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: [[10.1016/j.ymthe.2017.11.012]]). Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević https://wiki.fkkt.uni-lj.si/index.php?title=MBT_seminarji_2019&diff=15504 2019-04-13T11:11:41Z

<p>Nives Ražnjević: </p> <hr /> <div>'''Seznam seminarjev iz Molekularne biotehnologije v študijskem letu 2018/19'''<br /> <br /> Na tej strani je seznam odobrenih člankov za seminar ter povezave do člankov in do povzetkov, ki jih morate objaviti najkasneje do torka do polnoči v tednu, ko imate seminar (v četrtek). Angleški naslov prevedite tudi v slovenščino - to bo naslov povzetka, ki ga objavite na posebni strani, tako kot so to naredili kolegi pred vami (oz. predlani).<br /> <br /> Način vnosa:<br /> <br /> # The importance of ''Arabidopsis'' glutathione peroxidase 8 for protecting ''Arabidopsis'' plant and ''E. coli'' cells against oxidative stress (A. Gaber; GM Crops &amp; Food 5(1), 2014; http://dx.doi.org/10.4161/gmcr.26979) Pomen glutation peroksidaze 8 iz repnjakovca za zaščito rastline ''Arabidopsis thaliana'' in bakterije ''Escherichia coli'' pred oksidativnim stresom. Janez Novak<br /> (slovenski naslov povežite z novo stranjo, na kateri bo povzetek)<br /> <br /> <br /> '''Naslovi odobrenih člankov po temah:'''<br /> <br /> <br /> '''Gensko spremenjene rastline''' (14. marec)&lt;br&gt;<br /> # Production of functional human interleukin 37 using plants (N. Alqazlan, H. Diao, A. M. Jevnikar, and S. Ma; Plant Cell Rep. 38 (3), Mar. 2019; https://doi.org/10.1007/s00299-019-02377-2). [http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_funkcionalnega_%C4%8Dlove%C5%A1kega_IL37_v_rastlinah Proizvodnja funkcionalnega človeškega interlevkina 37 v rastlinah. Špela Malenšek]<br /> <br /> <br /> <br /> '''Gensko spremenjene živali''' (21. marec)&lt;br&gt;<br /> # Myopia disease mouse models: a missense point mutation (S673G) and a protein-truncating mutation of the ''Zfp644'' mimic human disease phenotype. (K. I. Szczerkowska ''et al.''; Cell Biosci. 9, 2019; https://doi.org/10.1186/s13578-019-0280-4). [http://wiki.fkkt.uni-lj.si/index.php/Mi%C5%A1ji_modeli_kratkovidnosti:_druga%C4%8Dnopomenska_to%C4%8Dkovna_mutacija_%28S673G%29_in_skraj%C5%A1evalna_mutacija_v_Zfp644_posnemata_fenotip_%C4%8Dlove%C5%A1ke_bolezni Mišji modeli kratkovidnosti: drugačnopomenska točkovna mutacija (S673G) in skrajševalna mutacija v ''Zfp644'' posnemata fenotip človeške bolezni] Rok Miklavčič<br /> # A chicken bioreactor for efficient production of functional cytokines (Herron L.R. ''et al.''; BMC Biotechnol. 18 (1), Dec. 2018; https://doi.org/10.1186/s12896-018-0495-1).[http://wiki.fkkt.uni-lj.si/index.php/Uporaba_kokošjega_bioreaktorja_za_učinkovito_proizvodnjo_funkcionalnih_citokinov Uporaba kokošjega bioreaktorja za učinkovito proizvodnjo funkcionalnih citokinov] Blaž Lebar<br /> # Influence of a growth hormone transgene on the genetic architecture of growth-related traits: A comparative analysis between transgenic and wild-type coho salmon (M. Kodama, K. A. Naish in R. H. Devlin; Evol Appl. 11(10), 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231474/). [http://wiki.fkkt.uni-lj.si/index.php/Vpliv_transgena_rastnega_hormona_na_genetsko_arhitekturo_lastnosti_povezanimi_z_rastjo Vpliv transgena rastnega hormona na genetsko arhitekturo lastnosti povezanimi z rastjo: primerjalna analiza transgenega srebrnega lososa in srebrnega lososa divjega tipa] Nuša Kelhar<br /> <br /> <br /> '''Okolje''' (4. april)&lt;br&gt;<br /> # Bioremediation of soil long-term contaminated with PAHs by algal-bacterial synergy of Chlorella sp. MM3 and Rhodococcus wratislaviensis strain 9 in slurry phase (S.R.Subashchandrabose, K. Venkateswarlu in K. Venkidusamy; Sci. Total Environ., 2019; https://www.sciencedirect.com/science/article/pii/S004896971835349X). [http://wiki.fkkt.uni-lj.si/index.php/Bioremediacija_zemlje%2C_dolgotrajno_kontaminirane_s_PAH Bioremediacija zemlje, dolgotrajno kontaminirane s policikličnimi aromatskimi ogljikovodiki z sinergijo alge Chlorella sp. MM3 in seva 9 bakterije Rhodococcus wratislaviensis v suspenziji.] Eva Rajh <br /> # Anaerobic degradation of xenobiotic isophthalate by the fermenting bacterium ''Syntrophorhabdus aromaticivorans'' (M. Junghare, D. Spiteller in B. Schink; ISME J, 2019; https://doi.org/10.1038/s41396-019-0348-5). [http://wiki.fkkt.uni-lj.si/index.php/Anaerobna_razgradnja_izoftalata_pri_fermentacijski_bakteriji_Syntrophorhabdus_aromaticivorans Anaerobna razgradnja ksenobiotika izoftalata pri fermentirajoči bakteriji ''Syntrophorhabdus aromaticivorans'']. Elvira Boršič<br /> # Biodegradation and toxicity of emerging contaminants: Isolation of an exopolysaccharide-producing ''Sphingomonas sp.'' for ionic liquids bioremediation. (M. Koutinas ''et al.''; J. Haz. Mat. 365, Mar. 2019; https://doi.org/10.1016/j.jhazmat.2018.10.059). [http://wiki.fkkt.uni-lj.si/index.php/Biorazgradnja_in_toksičnost_nastalih_produktov Biorazgradnja in toksičnost nastalih produktov: izolacija mikroorganizma Sphingomonas MKIV, ki proizvaja eksopolisaharide, za bioremediacijo ionskih tekočin] Katja Dolenc<br /> <br /> <br /> '''Terapevtski proteini in protitelesa''' (11. april)&lt;br&gt;<br /> # Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system (S. Murakami ''et al''; Scientific Reports 9, 2019; https://doi.org/10.1038/s41598-018-36691-8). [http://wiki.fkkt.uni-lj.si/index.php/U%C4%8Dinkovit_pristop_za_sintezo_funkcionalnega_IgG_z_uporabo_rekonstruiranega_brezceli%C4%8Dnega_sistema_za_sintezo_proteinov Učinkovit pristop za sintezo funkcionalnega IgG z uporabo rekonstruiranega brezceličnega sistema za sintezo proteinov.] Vida Štrancar<br /> # New therapeutic approach for targeting Hippo signalling pathway (L. Dominguez-Berrocal ''et al''; Scientific Reports 9(4771), 2019; https://www.nature.com/articles/s41598-019-41404-w). [http://wiki.fkkt.uni-lj.si/index.php/Nov_terapevtski_pristop_za_ciljanje_signalne_poti_Hippo Nov terapevtski pristop za ciljanje signalne poti Hippo.] Ana Halužan Vasle<br /> # Use of a design of experiments approach to optimise production of a recombinant antibody fragment in the periplasm of Escherichia coli: selection of signal peptide and optimal growth conditions (Kasli ''et al''. AMB Expr (2019) 9:5; https://doi.org/10.1186/s13568-018-0727-8). [http://wiki.fkkt.uni-lj.si/index.php/Uporaba_pristopa_načrtovanja_eksperimentov_za_optimizacijo_proizvodnje_rekombinantnega_fragmenta_protitelesa_v_periplazmi_Escherichie_coli:_izbira_signalnega_peptida_in_optimalnih_pogojev_rasti Uporaba pristopa načrtovanja eksperimentov za optimizacijo proizvodnje rekombinantnega fragmenta protitelesa v periplazmi ''Escherichie coli'': izbira signalnega peptida in optimalnih pogojev rasti.] Nina Mavec<br /> <br /> <br /> '''Diagnostiki in cepiva''' (18. april)&lt;br&gt;<br /> # A viral-vectored RSV vaccine induces long-lived humoral immunity in cotton rats (J. Grieves, Z. Yin, A. Garcia-Sastre et al.; Vaccine 36(26), 2018; https://doi.org/10.1016/j.vaccine.2018.04.089) Cepivo proti RSV, pripravljeno z virusnim vektorjem, inducira dolgotrajno humoralno imunost pri bombažnih podganah. Nina Kobe<br /> # Optimization of a multivalent peptide vaccine for nicotine addiction (D. F. Zeigler, R. Roque, C. H. Clegg; Vaccine 37(12), 2019; https://doi.org/10.1016/j.vaccine.2019.02.003) Optimizacija multivalentnega peptidnega cepiva za nikotinsko odvisnost. Iza Oblak<br /> # Design of a Type-1 Diabetes Vaccine Candidate Using Edible Plants Expressing a Major Autoantigen (E. Bertini ''et al''., Front. Plant Sci. 9, 2018 https://doi.org/10.3389/fpls.2018.00572) Načrtovnaje kandidatnega cepiva za diabetes tipa I s pomočjo užitnih rastlin, ki izražajo pomemben avtoantigen. Katarina Petra van Midden <br /> <br /> <br /> '''LMW učinkovine''' (25. april)&lt;br&gt; <br /> # Metabolic engineering of ''Escherichia coli'' for de novo biosynthesis of vitamin B&lt;sub&gt;12&lt;/sub&gt; (H. Fang ''et al.'', Nat Commun. 9(1), 2018 https://doi.org/10.1038/s41467-018-07412-6). Metabolno inženirstvo bakterije ''Escherichia coli'' za de novo sintezo vitamina B&lt;sub&gt;12&lt;/sub&gt;. Valentina Novak<br /> # David Titovšek<br /> # Bor Klančnik<br /> <br /> <br /> '''Male molekule in polimeri''' (9. maj)&lt;br&gt;<br /> # Primož Bembič<br /> # Karin Dobravc Škof<br /> # Jaka Kos<br /> <br /> <br /> '''Pretvorba biomase in bioenergenti''' (16. maj)&lt;br&gt;<br /> # Maksimiljan Adamek<br /> # Aljoša Marinko<br /> # Jošt Hočevar<br /> <br /> <br /> '''Novi pristopi v molekularni biotehnologiji''' (23. maj)&lt;br&gt;<br /> # Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells&lt;sub&gt;12&lt;/sub&gt; (Hung ''et al.'', Mol Ther. 7;26(2):456-467. doi: 10.1016/j.ymthe.2017.11.012. Inženirstvo protein-izločajočih plazmatki z homologno usmerjeno popravo v človeških B celicah &lt;sub&gt;12&lt;/sub&gt;. Nives Ražnjević<br /> # Katja Kunčič<br /> # Peter Pečan<br /> <br /> <br /> '''Rezervni termin''' (30. maj)&lt;br&gt;<br /> # Mia Žganjar<br /> # Ana Müller<br /> <br /> <br /> Nazaj na predmet [[Molekularna_biotehnologija]].</div>

Nives Ražnjević