A TALE nuclease architecture for efficient genome editing - Revision history

JernejMustar: /* A TALE nuclease architecture */

2015-01-11T15:48:11Z

A TALE nuclease architecture

← Older revision		Revision as of 15:48, 11 January 2015
Line 18:		Line 18:
	== A TALE nuclease architecture ==		== A TALE nuclease architecture ==

	As mentioned before, two studies from 2009 proposed the use of TALEs effectors for targeted genome engineering. These transcriptional activators originate from Xanthomonas plant pathogens. During pathogenesis, TALEs specifically bind and regulate plant gene expression, thus aiding bacterial infection [6]. A central repeat domain, which mediates DNA recognition, is located within TALE structure [1]. Each repeat unit comprises of 33 – 35 ~~amino~~, these specify one target base. The units base preference is determined by two crucial adjacent ~~amino acids~~, also called the “repeat variable di-residue” (RVD). Therefore, the sequence of specified units in central repeat domain determines the target sequence on genome. TALEs generated with new repeat combinations have been shown to recognize target sequences predicted by this code.		As mentioned before, two studies from 2009 proposed the use of TALEs effectors for targeted genome engineering. These transcriptional activators originate from Xanthomonas plant pathogens. During pathogenesis, TALEs specifically bind and regulate plant gene expression, thus aiding bacterial infection [6]. A central repeat domain, which mediates DNA recognition, is located within TALE structure [1]. Each repeat unit comprises of 33 – 35 aminoacids, these specify one target base. The units base preference is determined by two crucial adjacent aminoacids, also called the “repeat variable di-residue” (RVD). Therefore, the sequence of specified units in central repeat domain determines the target sequence on genome. TALEs generated with new repeat combinations have been shown to recognize target sequences predicted by this code.
	In the light of aforementioned advances the interest in potential use of this system in combination with nuclease was initiated. Subsequently, a TALE-nuclease chimeras (TALENs) was proposed, which would allow for site-specific genome cleavage. In selected paper, the advances in the field of TALEN’s technology are discussed. TALE activity in a mammalian cell environment for targeted regulation of episomal reporters and an endogenous gene is presented, resuming with the research of the minimal TALE region required for high-affinity DNA binding. Paper also includes practical application, showing modification of endogenous human genes NTF3 and CCR5 by the use of TALEN approach.		In the light of aforementioned advances the interest in potential use of this system in combination with nuclease was initiated. Subsequently, a TALE-nuclease chimeras (TALENs) was proposed, which would allow for site-specific genome cleavage. In selected paper, the advances in the field of TALEN’s technology are discussed. TALE activity in a mammalian cell environment for targeted regulation of episomal reporters and an endogenous gene is presented, resuming with the research of the minimal TALE region required for high-affinity DNA binding. Paper also includes practical application, showing modification of endogenous human genes NTF3 and CCR5 by the use of TALEN approach.

	== Retargeting a natural TALE to an endogenous mammalian sequence ==		== Retargeting a natural TALE to an endogenous mammalian sequence ==

JernejMustar: /* Retargeting a natural TALE to an endogenous mammalian sequence */

2015-01-11T15:46:42Z

Retargeting a natural TALE to an endogenous mammalian sequence

← Older revision		Revision as of 15:46, 11 January 2015
Line 23:		Line 23:
	== Retargeting a natural TALE to an endogenous mammalian sequence ==		== Retargeting a natural TALE to an endogenous mammalian sequence ==

	Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To ~~achive~~ this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection yielded one highly selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.		Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To achieve this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection yielded one highly selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.
	First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal promoter elements. TALE13 was modified to the extent of ~~recognising~~ proximal promoter region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which highlights its binding position on target. In order to test its activity, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These results revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.		First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal promoter elements. TALE13 was modified to the extent of recognizing proximal promoter region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which highlights its binding position on target. In order to test its activity, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These results revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.

	== Development of a TALE-nuclease architecture ==		== Development of a TALE-nuclease architecture ==

JernejMustar: /* Discussion */

2015-01-11T15:45:12Z

Discussion

← Older revision		Revision as of 15:45, 11 January 2015
Line 49:		Line 49:
	Presented TALEN architecture is in some characteristics similar to ZFNs. One example is the high cleavage activity of TALENs, which enables high gene modification efficiency, which is in some cases superior to the one of ZFNs. A shortcoming of TALEN architecture compared to ZFNs is its relative size. TALE repeats are 3 – 4 times larger than ZFPs, which may cause difficulty in delivery methods. Also, limitations due to high levels of TALE repeat homology may complicate the assembly of constructs. Future experiments will provide a fuller picture of pros and cons of the two platforms.		Presented TALEN architecture is in some characteristics similar to ZFNs. One example is the high cleavage activity of TALENs, which enables high gene modification efficiency, which is in some cases superior to the one of ZFNs. A shortcoming of TALEN architecture compared to ZFNs is its relative size. TALE repeats are 3 – 4 times larger than ZFPs, which may cause difficulty in delivery methods. Also, limitations due to high levels of TALE repeat homology may complicate the assembly of constructs. Future experiments will provide a fuller picture of pros and cons of the two platforms.
	Targeted endogenous gene regulation by TALE proteins is a useful tool in research and for biotechnology applications. We can use this technology in a broad sense, such as design and generation of transcriptional activators or repressors. Although TALEs were discovered as such, previous studies did not inspect this type of activity outside of their native plant context. The experiments shown in this paper suggest that TALEs can be used as transcriptional regulators.		Targeted endogenous gene regulation by TALE proteins is a useful tool in research and for biotechnology applications. We can use this technology in a broad sense, such as design and generation of transcriptional activators or repressors. Although TALEs were discovered as such, previous studies did not inspect this type of activity outside of their native plant context. The experiments shown in this paper suggest that TALEs can be used as transcriptional regulators.
	The use of SELEX assay for identification and characterization of TALEs has previously not been reported. By using this method, it was possible to assess the DNA binding preferences of natural and designed TALE proteins. Binding sites for TALE13 and four engineered TALEs were examined. On this basis, an observation that 73 out of 76 TALE repeats facilitate base preference of RVD. An interpretation of rare exceptions is proposed, facilitating further design and use of this system. Overall data collected can be used to examine previously acquired information on RVDs and thereby gaining more insight on mechanisms of action and selectivity. Findings that common RVDs such as NI, HD, NN and NG selectively bound selectively bound adenine, cytosine, guanine and thymine, respectively, are in accordance with previous knowledge. Pattern NN has shown relaxed specificity with substantial binding to a second base, adenine. NK is therefore more suitable for specificity, due to much stronger preference for guanine. This is a potential enhancement, proposed in this paper.		The use of SELEX assay for identification and characterization of TALEs has previously not been reported. By using this method, it was possible to assess the DNA binding preferences of natural and designed TALE proteins. Binding sites for TALE13 and four engineered TALEs were examined. On this basis, an observation that 73 out of 76 TALE repeats facilitate base preference of RVD. An interpretation of rare exceptions is proposed, facilitating further design and use of this system. Overall data collected can be used to examine previously acquired information on RVDs and thereby gaining more insight on mechanisms of action and selectivity. Findings that common RVDs such as NI, HD, NN and NG selectively bound selectively bound adenine, cytosine, guanine and thymine, respectively, are in accordance with previous knowledge. Pattern NN has shown relaxed specificity with substantial binding to a second base, adenine. NK is therefore more suitable for specificity, due to much stronger preference for guanine. This is a potential enhancement, proposed in this paper.

	== Expectations for the future ==		== Expectations for the future ==

JernejMustar: /* Expectations for the future */

2015-01-11T15:44:28Z

Expectations for the future

← Older revision		Revision as of 15:44, 11 January 2015
Line 55:		Line 55:
	As already mentioned, targeting the designated loci with ZFNs or TALENs is often difficult and time consuming, due to design and generation of new custom ZF or TALE array for each locus. This is not the case in most recent approach in genome editing, that is the use of CRISPR-Cas9 system. Expression of distinct gRNAs alongside Cas9 results in Cas9–gRNA complexes with distinct cleavage specificities [10]. This approach is exploited in multiplex genome editing. Still, there are features of this system which are inferior to elderly time-demanding ZFNs or TALENs.		As already mentioned, targeting the designated loci with ZFNs or TALENs is often difficult and time consuming, due to design and generation of new custom ZF or TALE array for each locus. This is not the case in most recent approach in genome editing, that is the use of CRISPR-Cas9 system. Expression of distinct gRNAs alongside Cas9 results in Cas9–gRNA complexes with distinct cleavage specificities [10]. This approach is exploited in multiplex genome editing. Still, there are features of this system which are inferior to elderly time-demanding ZFNs or TALENs.
	One of those features is size of gene constructs, which is a limitation in field of gene delivery. Cas9 is the size of about 160 kDa, whereas a typical 17,5 repeat TALEN corresponds to 105 kDa. By far the smallest are ZFNs, wherein typical four finger ZFN has about 40 kDa [10]. It is likely for this compact nature to be beneficial for certain experiments.		One of those features is size of gene constructs, which is a limitation in field of gene delivery. Cas9 is the size of about 160 kDa, whereas a typical 17,5 repeat TALEN corresponds to 105 kDa. By far the smallest are ZFNs, wherein typical four finger ZFN has about 40 kDa [10]. It is likely for this compact nature to be beneficial for certain experiments.
	As for specificity, the number of bases for modification in Cas9 system is restricted to 20 and is somewhat limited ~~with~~ the rule of three guanine bases flanking the target site (GN19NGG) [10]. The main constriction of ZF arrays is a scarce set of fingers, resulting in inability to target certain triplets. In addition, some ZFs targeting GNN seem to be superior to other ZFs, contributing to limitations. TALE target specificity therefore seems superior, with capabilities of targeting up to 24 bases. For Cas9 system, off target studies in mammalian environment are yet to be conducted [10].		As for specificity, the number of bases for modification in Cas9 system is restricted to 20 and is somewhat limited by the rule of three guanine bases flanking the target site (GN19NGG) [10]. The main constriction of ZF arrays is a scarce set of fingers, resulting in inability to target certain triplets. In addition, some ZFs targeting GNN seem to be superior to other ZFs, contributing to limitations. TALE target specificity therefore seems superior, with capabilities of targeting up to 24 bases. For Cas9 system, off target studies in mammalian environment are yet to be conducted [10].
	It is likely that distinct features will lead to differential use of genome editing systems for distinct applications in the fields of plant science, therapeutic sciences and biotechnology applications.		It is likely that distinct features will lead to differential use of genome editing systems for distinct applications in the fields of plant science, therapeutic sciences and biotechnology applications.

	== Literature ==		== Literature ==

JernejMustar: /* Retargeting a natural TALE to an endogenous mammalian sequence */

2015-01-11T15:42:29Z

Retargeting a natural TALE to an endogenous mammalian sequence

← Older revision		Revision as of 15:42, 11 January 2015
Line 23:		Line 23:
	== Retargeting a natural TALE to an endogenous mammalian sequence ==		== Retargeting a natural TALE to an endogenous mammalian sequence ==

	Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To achive this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection ~~yield~~ one ~~higly~~ selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.		Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To achive this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection yielded one highly selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.
	First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal ~~promotor~~ elements. TALE13 was modified to the extent of recognising proximal ~~promotor~~ region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which ~~highlits~~ its binding position on target. In order to test its ~~aktivity~~, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These results revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.		First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal promoter elements. TALE13 was modified to the extent of recognising proximal promoter region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which highlights its binding position on target. In order to test its activity, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These results revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.

	== Development of a TALE-nuclease architecture ==		== Development of a TALE-nuclease architecture ==

JernejMustar at 15:36, 11 January 2015

2015-01-11T15:36:30Z

← Older revision		Revision as of 15:36, 11 January 2015
Line 1:		Line 1:
	(Jernej Mustar)		(Jernej Mustar)

	Summarized from Miller, J. C. ''et al''. A TALE nuclease architecture for efficient genome editing. ''Nat. Biotechnol.'', vol. 29, pp. 143–148. Feb. 2011.		Summarized from Miller, J. C. ''et al''. A TALE nuclease architecture for efficient genome editing. ''Nat. Biotechnol.'', vol. 29, pp. 143–148. Feb. 2011.

JernejMustar at 15:36, 11 January 2015

2015-01-11T15:36:20Z

← Older revision		Revision as of 15:36, 11 January 2015
Line 1:		Line 1:
			(Jernej Mustar)
	Summarized from Miller, J. C. ''et al''. A TALE nuclease architecture for efficient genome editing. ''Nat. Biotechnol.'', vol. 29, pp. 143–148. Feb. 2011.		Summarized from Miller, J. C. ''et al''. A TALE nuclease architecture for efficient genome editing. ''Nat. Biotechnol.'', vol. 29, pp. 143–148. Feb. 2011.

JernejMustar: /* Brief history of genome editing */

2015-01-11T15:35:10Z

Brief history of genome editing

← Older revision		Revision as of 15:35, 11 January 2015
Line 10:		Line 10:
	== Brief history of genome editing ==		== Brief history of genome editing ==

	Past studies in the field of genome editing were perusing a common goal, t. i. to obtain high specificity, which would limit the target affects [2]. The first engineered technology, based on nuclease activity, was introduced in 1991 by Pavletich and Pabo in the journal Science [2]. This technology by the name of Zinc Finger nuclease was a great breakthrough and was later on frequently applied for genome targeting. It is based on the use of cleavage domain of restriction enzyme FokI, which is linked with designated zinc finger protein (ZFP), which is responsible for targeting. By the use of different combinations of ZFP domains the selectivity for single genomic cleavage event is achieved. ~~The~~ use of nuclease domains with monomeric activity there is a high probability of off-target events. This can be avoided by the use of a pair of ZFNs with ~~distinct~~ FokI domains that are obligate heterodimers [3]. Overtime, reports of problems and drawbacks of this technology were reported, such as targeting problem for certain triplets and reduced specificity, suffered by interactions within a zinc finger array [2].		Past studies in the field of genome editing were perusing a common goal, t. i. to obtain high specificity, which would limit the target affects [2]. The first engineered technology, based on nuclease activity, was introduced in 1991 by Pavletich and Pabo in the journal Science [2]. This technology by the name of Zinc Finger nuclease was a great breakthrough and was later on frequently applied for genome targeting. It is based on the use of cleavage domain of restriction enzyme FokI, which is linked with designated zinc finger protein (ZFP), which is responsible for targeting. By the use of different combinations of ZFP domains the selectivity for single genomic cleavage event is achieved. With the use of nuclease domains with monomeric activity there is a high probability of off-target events. This can be avoided by the use of a distinct pair of ZFNs with FokI domains that are obligate heterodimers [3]. Overtime, reports of problems and drawbacks of this technology were reported, such as targeting problem for certain triplets and reduced specificity, suffered by interactions within a zinc finger array [2].
	The next technology, which emerged in 2009, is called transcription activator-like effector nuclease (TALENs). These are fusion proteins, where nuclease domain is associated with TAL effector DNA binding domain. This approach may be better in certain aspects, for example it enables a larger spectrum of targets and ~~it’s simpler and~~ more practical than ZFNs. In 2011, TALENs was proclaimed as “Method of the Year” by the journal Nature methods. The details of this system and molecular mechanisms of function are described later on.		The next technology, which emerged in 2009, is called transcription activator-like effector nuclease (TALENs). These are fusion proteins, where nuclease domain is associated with TAL effector DNA binding domain. This approach may be better in certain aspects, for example it enables a larger spectrum of targets and is more practical than ZFNs. In 2011, TALENs was proclaimed as “Method of the Year” by the journal Nature methods. The details of this system and molecular mechanisms of function are described later on.
	We probably all at least heard if not read about latest breakthrough in the field of genome editing, which is CRISPR-Cas9 system. The discovery was somewhat fortuity, because researchers were studying how bacteria defend against phages and foreign plasmids. The paper was published in August 2012 in journal Science [4]. A bacterial nuclease called Cas9 is capable of altering genomes of invading viruses, consequently inactivating them. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA loci that contain short sequence repetitions, which can be transcribed, processed and then used to guide nuclease to a specific site [5]. This is in contrast with TALEN and Zinc Finger methods, where the need to prepare customized nuclease for each target remains.		We probably all at least heard if not read about latest breakthrough in the field of genome editing, which is CRISPR-Cas9 system. The discovery was somewhat fortuity, because researchers were studying how bacteria defend against phages and foreign plasmids. The paper was published in August 2012 in journal Science [4]. A bacterial nuclease called Cas9 is capable of altering genomes of invading viruses, consequently inactivating them. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA loci that contain short sequence repetitions, which can be transcribed, processed and then used to guide nuclease to a specific site [5]. This is in contrast with TALEN and Zinc Finger methods, where the need to prepare customized nuclease for each target remains.

	== A TALE nuclease architecture ==		== A TALE nuclease architecture ==

JernejMustar: /* Introduction to genome editing */

2015-01-11T15:30:57Z

Introduction to genome editing

← Older revision		Revision as of 15:30, 11 January 2015
Line 3:		Line 3:
	== Introduction to genome editing ==		== Introduction to genome editing ==

	Genome editing is a type of genetic engineering, where an organisms DNA is manipulated by altering; inserting, removing or replacing nucleotides, therefore changing its genome. This process is performed by the use of immensely useful tool, t. i. engineered nucleases, which are capable of cutting both strands of DNA or nicking single strand. Resulting sites are detected and repaired by endogenous repair mechanisms, which include homologous recombination (HR) or non-homologous end joining (NHEJ). The latter is error prone, therefore it is likely for mutations to occur in repair site. These repair pathways are highly conserved in diverse cell types, which is beneficial for the use of genome editing technologies. As a result, various cell types have been engineered by the use of ~~potable~~ cleavage-based technologies, including human stem cells [1].		Genome editing is a type of genetic engineering, where an organisms DNA is manipulated by altering; inserting, removing or replacing nucleotides, therefore changing its genome. This process is performed by the use of immensely useful tool, t. i. engineered nucleases, which are capable of cutting both strands of DNA or nicking single strand. Resulting sites are detected and repaired by endogenous repair mechanisms, which include homologous recombination (HR) or non-homologous end joining (NHEJ). The latter is error prone, therefore it is likely for mutations to occur in repair site. These repair pathways are highly conserved in diverse cell types, which is beneficial for the use of genome editing technologies. As a result, various cell types have been engineered by the use of portable cleavage-based technologies, including human stem cells [1].

	Recently, there has been tremendous development in the field of synthetic biology and genome editing technology. Virtually any gene can be manipulated in diverse range of organisms. Most common approach is the use of nuclease, which can be manipulated to target specific sequences of genome. This can be achieved by exploitation of sequence-specific DNA-binding domains (ZFNs, TALENs) or by the use of complementary guide RNA (CRISPR-Cas9 system).		Recently, there has been tremendous development in the field of synthetic biology and genome editing technology. Virtually any gene can be manipulated in diverse range of organisms. Most common approach is the use of nuclease, which can be manipulated to target specific sequences of genome. This can be achieved by exploitation of sequence-specific DNA-binding domains (ZFNs, TALENs) or by the use of complementary guide RNA (CRISPR-Cas9 system).

JernejMustar at 15:28, 11 January 2015

2015-01-11T15:28:31Z

← Older revision		Revision as of 15:28, 11 January 2015
Line 22:		Line 22:

	Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To achive this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection yield one higly selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.		Firstly, a suitable TAL effector for this study was chosen. Criteria for TALE selection was based on specificity and empirical evidence. The group was searching for natural TALE with high specificity for target sequence and with confirmed activity in mammalian cells. To achive this goal, PCR amplifications for several TALEs using Xanthomonas axonopodis pathovar citri genomic DNA were performed. Native TALE-associated sequences which allow transport into plant cells were excluded. Protein products of acquired coding regions were characterized by a SELEX assay using target of interest. Outcome of this selection yield one higly selective candidate, identified as TALE13. When combined with VP-16 activation domain, a 70-fold induction of reporter gene expression rate was observed in HEK293 cells. Based on these results, TALE13 was chosen for further work and design.
	First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal promotor elements. TALE13 was modified to the extent of recognising proximal promotor region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which highlits its binding position on target. In order to test its aktivity, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These ~~resoults~~ revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.		First goal was to demonstrate that chosen candidate was appropriate for mammalian gene regulation. Chosen target was NTF3 gene, which encodes a secreted nerve growth factor that has therapeutic potential for neurodegenerative diseases. Wild type TALE usually contain 18 repeats, by which they target specific sequence and bind to proximal promotor elements. TALE13 was modified to the extent of recognising proximal promotor region of NTF3 by replacing the 18 wild-type repeat units with appropriate alternatives. This altered TALE was named NT-L (meaning NTF3 Left), which highlits its binding position on target. In order to test its aktivity, the truncated version of NT-L was fused with VP-16 and expressed in human HEK293 cells. By measuring the activity of endogenous NTF3 locus, a strong induction (over 20 fold) in both NTF3 transcript and protein product was detected. The subsequent SELEX analysis revealed high specificity. These results revealed, that NT-L variant is a viable candidate for endogenous mammalian gene regulation.

	== Development of a TALE-nuclease architecture ==		== Development of a TALE-nuclease architecture ==