https://wiki.fkkt.uni-lj.si/index.php?action=history&feed=atom&title=Ovalbumini 2026-04-09T04:33:53Z Revision history for this page on the wiki MediaWiki 1.39.3 https://wiki.fkkt.uni-lj.si/index.php?title=Ovalbumini&diff=6906&oldid=prev 2012-03-14T15:43:50Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:43, 14 March 2012</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Opis ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Opis ==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>&#039;&#039;&#039;Ovalbumini&#039;&#039;&#039; (lat. &#039;&#039;ovi albumen&#039;&#039; iz &#039;&#039;ovum&#039;&#039; - jajce in &#039;&#039;albumen&#039;&#039; - beljak) so <del style="font-weight: bold; text-decoration: none;">glavni [[kristalni </del>proteini<del style="font-weight: bold; text-decoration: none;">]] ali [[albumini]], ki se nahajajo v jajčnem beljaku</del>. Ovalbumin je [[fosfoprotein]] sestavljen iz 386 aminokislin z eno N-vezano oligosaharidno verigo. Ima kristalno (tridimenzionalno) strukturo in ga uvrščamo med [[<del style="font-weight: bold; text-decoration: none;">serapin</del>]]e, ki vkjučujejo tudi kjučne zaviralce serinske proteaze človeške plazme, kot tudi <del style="font-weight: bold; text-decoration: none;">nezavirajoče </del>homologe, kot so globulin in angiotenzinogen. Funkcija ovalbuminov je neznana, sicer se domneva da so biološke rezerve kovinskih ionov in aminokislin, ki jih uporabljajo organizmi in <del style="font-weight: bold; text-decoration: none;">se nahajajo </del>v jajčnem beljaku, mleku in semenih rastlin.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>&#039;&#039;&#039;Ovalbumini&#039;&#039;&#039; (lat. &#039;&#039;ovi albumen&#039;&#039; iz &#039;&#039;ovum&#039;&#039; - jajce in &#039;&#039;albumen&#039;&#039; - beljak) so <ins style="font-weight: bold; text-decoration: none;">poglavitni </ins>proteini <ins style="font-weight: bold; text-decoration: none;">jajčnega beljaka</ins>. Ovalbumin je [[fosfoprotein]] sestavljen iz 386 aminokislin z eno N-vezano oligosaharidno verigo. Ima <ins style="font-weight: bold; text-decoration: none;">znano </ins>kristalno (tridimenzionalno) strukturo in ga uvrščamo med [[<ins style="font-weight: bold; text-decoration: none;">serpin</ins>]]e, ki vkjučujejo tudi kjučne zaviralce serinske proteaze človeške plazme, kot tudi <ins style="font-weight: bold; text-decoration: none;">neinhibitorne </ins>homologe, kot so globulin in angiotenzinogen. Funkcija ovalbuminov je neznana, sicer se domneva da so biološke rezerve kovinskih ionov in aminokislin, ki jih uporabljajo organizmi in <ins style="font-weight: bold; text-decoration: none;">so shranjene </ins>v jajčnem beljaku, mleku in semenih rastlin.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Lastnosti ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Lastnosti ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Molekulska masa ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Molekulska masa ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Molekulska masa ovalbuminov je od 44<del style="font-weight: bold; text-decoration: none;">.</del>0-45.<del style="font-weight: bold; text-decoration: none;">0 kDA</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Molekulska masa ovalbuminov je od 44<ins style="font-weight: bold; text-decoration: none;">,</ins>0-45<ins style="font-weight: bold; text-decoration: none;">,0 kDa</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Izoelektrična točka ===  </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Izoelektrična točka ===  </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>pI 4<del style="font-weight: bold; text-decoration: none;">.</del>6</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>pI <ins style="font-weight: bold; text-decoration: none;">= </ins>4<ins style="font-weight: bold; text-decoration: none;">,</ins>6</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Biološka funkcija ===  </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Biološka funkcija ===  </div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Funkcija ovalbuminov je neznana. Domneva se da ima vlogo [[shranjevalnega proteina]].</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Funkcija ovalbuminov je neznana. Domneva se da ima vlogo [[shranjevalnega proteina]].</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumine uvrščamo v družino <del style="font-weight: bold; text-decoration: none;">serapinov proteaznih [[inhibitor]]jev</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumine uvrščamo v družino <ins style="font-weight: bold; text-decoration: none;">serpinov, inhibitorjev serinskih proteaz</ins>.</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Aktivno mesto: 352-353</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Aktivno mesto: <ins style="font-weight: bold; text-decoration: none;">aminokislinska ostanka </ins>352-353</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Struktura ===  </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Struktura ===  </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Tridimenzionalna-kristalna struktura</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Tridimenzionalna-kristalna struktura <ins style="font-weight: bold; text-decoration: none;">je znana.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Stabilnost ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Stabilnost ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Pretvori se v [[S-ovalbumin]] pri segrevanju in shranjevanju. 5% S-oblike se nahaja v svežih jajcih in 81% v jajcih, ki so skladiščena 6 mesecev. S-obilka je bolj odporna na toploto in težje denaturira (izgubi nativno obliko).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Pretvori se v [[S-ovalbumin]] pri segrevanju in shranjevanju. 5 % S-oblike se nahaja v svežih jajcih in 81 % v jajcih, ki so skladiščena 6 mesecev. S-obilka je bolj odporna na toploto in težje denaturira (izgubi nativno obliko).</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Emulzifikacija ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Emulzifikacija ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Emulzifikacijske lastnosti ovalbuminov so odvisne od pH<del style="font-weight: bold; text-decoration: none;">-ja</del>, prisotnosti soli, koncentracije <del style="font-weight: bold; text-decoration: none;">disperzije </del>proteinov in volumna oljne faze. Emulzifikacijska aktivnost ovalbuminov je večja v kislem območju pH.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Emulzifikacijske lastnosti ovalbuminov so odvisne od pH, prisotnosti soli, koncentracije <ins style="font-weight: bold; text-decoration: none;">dispergiranih </ins>proteinov in volumna oljne faze. Emulzifikacijska aktivnost ovalbuminov je večja v kislem območju pH.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Uporaba ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Uporaba ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Rekombinantni protein ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Rekombinantni protein ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumin <del style="font-weight: bold; text-decoration: none;">se uporablja </del>kot [[rekombinantni protein]] <del style="font-weight: bold; text-decoration: none;">in sicer se izraža</del>:</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumin <ins style="font-weight: bold; text-decoration: none;">so </ins>kot [[rekombinantni protein]] <ins style="font-weight: bold; text-decoration: none;">pripravili v</ins>:</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>          </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>          </div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>- <del style="font-weight: bold; text-decoration: none;">v Escherichii Coli</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>- <ins style="font-weight: bold; text-decoration: none;">bakteriji Escherichia coli</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>- <del style="font-weight: bold; text-decoration: none;">v </del>mišjih celicah</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>- mišjih celicah</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>-<del style="font-weight: bold; text-decoration: none;">v </del>človeških <del style="font-weight: bold; text-decoration: none;">karcinogenih </del>celicah dojk</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>- človeških <ins style="font-weight: bold; text-decoration: none;">rakavih </ins>celicah dojk</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Imunologija ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Imunologija ===</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumini se pogosto uporabljajo kot sprožilci kemijskih reakcij pri različnih imunoloških testih.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Ovalbumini se pogosto uporabljajo kot sprožilci kemijskih reakcij pri različnih imunoloških testih.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Elektroforeza ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Elektroforeza ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Ovalbumini </del>kokošjih jajc se <del style="font-weight: bold; text-decoration: none;">uporabljajo </del>tudi kot <del style="font-weight: bold; text-decoration: none;">označevalci </del>molekulske <del style="font-weight: bold; text-decoration: none;">teže </del>za kalibriranje elektroforenznega gela.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Ovalbumin iz </ins>kokošjih jajc se <ins style="font-weight: bold; text-decoration: none;">uporablja </ins>tudi kot <ins style="font-weight: bold; text-decoration: none;">označevalec </ins>molekulske <ins style="font-weight: bold; text-decoration: none;">mase </ins>za kalibriranje elektroforenznega gela.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Viri ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Viri ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[Storage protein, Wikipeida:</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[Storage protein, Wikipeida:</div></td></tr> </table>

MDolinar https://wiki.fkkt.uni-lj.si/index.php?title=Ovalbumini&diff=6721&oldid=prev 2012-01-03T21:57:03Z

<p>New page: == Opis == &#039;&#039;&#039;Ovalbumini&#039;&#039;&#039; (lat. &#039;&#039;ovi albumen&#039;&#039; iz &#039;&#039;ovum&#039;&#039; - jajce in &#039;&#039;albumen&#039;&#039; - beljak) so glavni <a href="/index.php?title=Kristalni_proteini&amp;action=edit&amp;redlink=1" class="new" title="Kristalni proteini (page does not exist)">kristalni proteini</a> ali <a href="/index.php/Albumini" title="Albumini">albumini</a>, ki se nahajajo v jajčnem beljaku. Ovalbum...</p> <p><b>New page</b></p><div>== Opis ==<br /> &#039;&#039;&#039;Ovalbumini&#039;&#039;&#039; (lat. &#039;&#039;ovi albumen&#039;&#039; iz &#039;&#039;ovum&#039;&#039; - jajce in &#039;&#039;albumen&#039;&#039; - beljak) so glavni [[kristalni proteini]] ali [[albumini]], ki se nahajajo v jajčnem beljaku. Ovalbumin je [[fosfoprotein]] sestavljen iz 386 aminokislin z eno N-vezano oligosaharidno verigo. Ima kristalno (tridimenzionalno) strukturo in ga uvrščamo med [[serapin]]e, ki vkjučujejo tudi kjučne zaviralce serinske proteaze človeške plazme, kot tudi nezavirajoče homologe, kot so globulin in angiotenzinogen. Funkcija ovalbuminov je neznana, sicer se domneva da so biološke rezerve kovinskih ionov in aminokislin, ki jih uporabljajo organizmi in se nahajajo v jajčnem beljaku, mleku in semenih rastlin.<br /> == Lastnosti ==<br /> === Molekulska masa ===<br /> Molekulska masa ovalbuminov je od 44.0-45.0 kDA<br /> === Izoelektrična točka === <br /> pI 4.6<br /> === Biološka funkcija === <br /> Funkcija ovalbuminov je neznana. Domneva se da ima vlogo [[shranjevalnega proteina]].<br /> Ovalbumine uvrščamo v družino serapinov proteaznih [[inhibitor]]jev.<br /> Aktivno mesto: 352-353<br /> === Struktura === <br /> Tridimenzionalna-kristalna struktura<br /> === Stabilnost ===<br /> Pretvori se v [[S-ovalbumin]] pri segrevanju in shranjevanju. 5% S-oblike se nahaja v svežih jajcih in 81% v jajcih, ki so skladiščena 6 mesecev. S-obilka je bolj odporna na toploto in težje denaturira (izgubi nativno obliko).<br /> === Emulzifikacija ===<br /> Emulzifikacijske lastnosti ovalbuminov so odvisne od pH-ja, prisotnosti soli, koncentracije disperzije proteinov in volumna oljne faze. Emulzifikacijska aktivnost ovalbuminov je večja v kislem območju pH.<br /> == Uporaba ==<br /> === Rekombinantni protein ===<br /> Ovalbumin se uporablja kot [[rekombinantni protein]] in sicer se izraža:<br /> <br /> - v Escherichii Coli<br /> <br /> - v mišjih celicah<br /> <br /> -v človeških karcinogenih celicah dojk<br /> === Imunologija ===<br /> Ovalbumini se pogosto uporabljajo kot sprožilci kemijskih reakcij pri različnih imunoloških testih.<br /> === Elektroforeza ===<br /> Ovalbumini kokošjih jajc se uporabljajo tudi kot označevalci molekulske teže za kalibriranje elektroforenznega gela.<br /> == Viri ==<br /> [Storage protein, Wikipeida:<br /> http://www.food-allergens.de/symposium-vol1%281%29/data/egg-white/gald2.htm]<br /> <br /> [Merriam-Webster: An encyclopedia britannica company:<br /> http://www.merriam-webster.com/dictionary/ovalbumin]<br /> <br /> [J. Agric:Food Chem. stran 443<br /> http://pubs.acs.org/doi/abs/10.1021/jf00003a003]<br /> <br /> [Crystal structure of ovalbumin as a model for the reactive centre of serpins, letters to nature, npg:<br /> http://www.nature.com/nature/journal/v347/n6288/abs/347099a0.html]<br /> <br /> [Your dictionary, Love to know:<br /> http://www.yourdictionary.com/ovalbumin]<br /> <br /> [Ovalbumin, Wikipedia:<br /> http://en.wikipedia.org/wiki/Ovalbumin]<br /> <br /> == Zunanje povezave ==<br /> <br /> [Jajce:<br /> http://www.talkallergy.com/image_01/clip_art/eggs.jpg (slika)]<br /> <br /> [Ovalbumin:<br /> http://static2.trinklaune.de/wp-content/uploads/2011/11/Ovalbumin.png (slika)]<br /> <br /> [Ovalbumin, neova technologies, A Bioseutica olc Company:<br /> http://www.neovatech.com/pdfs/ovoproducts/ovoproducts_ovalbumin.pdf]<br /> <br /> [DQ, Ovalbumin (D-12053), Molecular probes:<br /> http://probes.invitrogen.com/media/pis/mp12053.pdf]<br /> [[Category:LEX]]</div>

Marko Ikonić