Metode za gojenje solinskih in morskih mikroorganizmov: Difference between revisions

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Gojišča za cianobakterije:
Gojišča za cianobakterije:
[[Image:gojisca.png]] [Rosmarie Rippka,  Josette Deruelles,  John B. Waterbury,  Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]
Povzeto po članku: Rosmarie Rippka,  Josette Deruelles,  John B. Waterbury,  Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4


Gojišče MN
'''Gojišče MN'''
NaCl
''[g/liter gojišča]''
MgCI, . 6H20
*NaNO3 -> 0.75
KC1
*K2HPO4.3H2O -> 0.02
NaNO,
*MgS04.7H2O -> 0.038
KaHPO4.3HzO
*CaC12.2H20 -> 0.018
MgS04.7HzO
*Citric acid -> 0.003
CaC1,. 2H20
*Ferric ammonium citrate -> 0.003
Citric acid
*EDTA -> 0.0005
Ferric ammonium citrate
*Na2C03 -> 0.02
EDTA (disodium magnesium salt)
*Trace metal mix -> 1ml '''(*)'''
Na2C03
*Sea water -> 750 ml
Trace metal mix A5 + Co*
*Deionized water -> 250 ml
Sea water
Deionized water


-
'''Gojišče ASN-I11'''
0.75
''[g/liter gojišča]''
0.02
*NaCl -> 25.0
0.038
*MgCI2.6H20 -> 2.0
0.018
*KC1 -> 0.5
0.003
*NaNO3 -> 0.75
0.003
*K2HPO4.3H2O -> 0.02
0.0005
*MgS04.7H2O -> 3.5
0.02
*CaC12.2H20 -> 0.5
1 ml1-1
*Citric acid -> 0.003
750 ml
*Ferric ammonium citrate -> 0.003
250 ml
*EDTA -> 0.0005
25.0
*Na2C03 -> 0.02
*Trace metal mix -> 1 ml '''(*)'''
*Deionized water -> 1000 ml


ASN-I11
'''(*) Trace metal mix:'''
2.0
''[g/liter]''
0.5
*H3BO3 -> 2.86
0-75
*MnCI2.4H20 -> 1.81
0.02
*ZnSO4.7H2O -> 0.222
3.5
*Na2MoO4.2H20 -> 0.390
0.5
*CuSO4.5H20 -> 0.079
0.003
*Co(N03)2.6H20 -> 0.00494
0.003
04005
0.02
1 ml1-1
1000 ml

Latest revision as of 14:17, 19 May 2018

Gojišča za cianobakterije: Povzeto po članku: Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4

Gojišče MN [g/liter gojišča]

  • NaNO3 -> 0.75
  • K2HPO4.3H2O -> 0.02
  • MgS04.7H2O -> 0.038
  • CaC12.2H20 -> 0.018
  • Citric acid -> 0.003
  • Ferric ammonium citrate -> 0.003
  • EDTA -> 0.0005
  • Na2C03 -> 0.02
  • Trace metal mix -> 1ml (*)
  • Sea water -> 750 ml
  • Deionized water -> 250 ml

Gojišče ASN-I11 [g/liter gojišča]

  • NaCl -> 25.0
  • MgCI2.6H20 -> 2.0
  • KC1 -> 0.5
  • NaNO3 -> 0.75
  • K2HPO4.3H2O -> 0.02
  • MgS04.7H2O -> 3.5
  • CaC12.2H20 -> 0.5
  • Citric acid -> 0.003
  • Ferric ammonium citrate -> 0.003
  • EDTA -> 0.0005
  • Na2C03 -> 0.02
  • Trace metal mix -> 1 ml (*)
  • Deionized water -> 1000 ml

(*) Trace metal mix: [g/liter]

  • H3BO3 -> 2.86
  • MnCI2.4H20 -> 1.81
  • ZnSO4.7H2O -> 0.222
  • Na2MoO4.2H20 -> 0.390
  • CuSO4.5H20 -> 0.079
  • Co(N03)2.6H20 -> 0.00494
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