Reverzna transkriptaza: Difference between revisions
Miha Virant (talk | contribs) No edit summary |
Miha Virant (talk | contribs) No edit summary |
||
| Line 11: | Line 11: | ||
Reakcija, ki jo katalizira: | Reakcija, ki jo katalizira: | ||
deoxynucleoside triphosphate + < | deoxynucleoside triphosphate + DNA<sub>n</sub> = diphosphate + DNA<sub>n+1</sub> | ||
== Viri == | == Viri == | ||
* <sup>[1]</sup> [http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html] | * <sup>[1]</sup> [http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/7/7/49.html] | ||
* <sup>[2]</sup> [http://pfam.sanger.ac.uk/family?acc=PF00078 http://pfam.sanger.ac.uk/family?acc=PF00078] | * <sup>[2]</sup> [http://pfam.sanger.ac.uk/family?acc=PF00078 http://pfam.sanger.ac.uk/family?acc=PF00078] | ||
Revision as of 14:00, 12 December 2010
Reverzna transkriptaza je encim (EC 2.7.7.49), ki spada v razred transferaz. Katalizira podaljševanje DNA verige na 3'- koncu na osnovi RNA matrice, torej omogoča prepis RNA na DNA (en. RNA-directed DNA polymerase). Za začetek potrebuje že obstoječ RNA ali DNA primer – ne more ustvariti verige od začetka ("de novo" ).[1] Prepis poteka tako, da na obstoječi primer dodaja posamezne nukleotide, ki so komplementarni RNA matrici, torej katalizira proces, ki poteka v nasprotni smeri kakor transkripcija
Odkrila sta jo neodvisno drug od drugega Howard Temin, University of Wisconsin-Madison in David Baltimore, MIT (Massachusetts Institute of Technology). Skupaj z Renatom Dulbeccom so za ta dosežek leta 1975 prejeli Nobelovo nagrado.
Katalizira procese v: [2]
- retrovirusih
- hepadnavirusih in caulimovirusih
- retrotransposonih
- bakterijki msDNA
- intronih tipa II
Reakcija, ki jo katalizira: deoxynucleoside triphosphate + DNAn = diphosphate + DNAn+1
