Metode za gojenje solinskih in morskih mikroorganizmov: Difference between revisions
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| Line 12: | Line 12: | ||
*EDTA -> 0.0005 | *EDTA -> 0.0005 | ||
*Na2C03 -> 0.02 | *Na2C03 -> 0.02 | ||
*Trace metal mix -> 1ml | *Trace metal mix -> 1ml (*) | ||
*Sea water -> 750 ml | *Sea water -> 750 ml | ||
*Deionized water -> 250 ml | *Deionized water -> 250 ml | ||
| Line 18: | Line 18: | ||
Gojišče ASN-I11 | Gojišče ASN-I11 | ||
[g/liter gojišča] | [g/liter gojišča] | ||
NaCl -> 25.0 | *NaCl -> 25.0 | ||
MgCI2.6H20 -> 2.0 | *MgCI2.6H20 -> 2.0 | ||
KC1 -> 0.5 | *KC1 -> 0.5 | ||
NaNO3 -> 0.75 | *NaNO3 -> 0.75 | ||
K2HPO4.3H2O -> 0.02 | *K2HPO4.3H2O -> 0.02 | ||
MgS04.7H2O -> 3.5 | *MgS04.7H2O -> 3.5 | ||
CaC12.2H20 -> 0.5 | *CaC12.2H20 -> 0.5 | ||
Citric acid -> 0.003 | *Citric acid -> 0.003 | ||
Ferric ammonium citrate -> 0.003 | *Ferric ammonium citrate -> 0.003 | ||
EDTA -> 0.0005 | *EDTA -> 0.0005 | ||
Na2C03 -> 0.02 | *Na2C03 -> 0.02 | ||
Trace metal mix -> 1 ml | *Trace metal mix -> 1 ml (*) | ||
Deionized water -> 1000 ml | *Deionized water -> 1000 ml | ||
*Trace metal mix: | (*) Trace metal mix: | ||
[g/liter] | [g/liter] | ||
H3BO3 -> 2.86 | *H3BO3 -> 2.86 | ||
MnCI2.4H20 -> 1.81 | *MnCI2.4H20 -> 1.81 | ||
ZnSO4.7H2O -> 0.222 | *ZnSO4.7H2O -> 0.222 | ||
Na2MoO4.2H20 -> 0.390 | *Na2MoO4.2H20 -> 0.390 | ||
CuSO4.5H20 -> 0.079 | *CuSO4.5H20 -> 0.079 | ||
Co(N03)2.6H20 -> 0.00494 | *Co(N03)2.6H20 -> 0.00494 | ||
Revision as of 14:16, 19 May 2018
Gojišča za cianobakterije: File:Gojisca.png [Rosmarie Rippka, Josette Deruelles, John B. Waterbury, Michael Herdman, Roger Y. Stanier. 1979. Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 111: 4]
Gojišče MN [g/liter gojišča]
- NaNO3 -> 0.75
- K2HPO4.3H2O -> 0.02
- MgS04.7H2O -> 0.038
- CaC12.2H20 -> 0.018
- Citric acid -> 0.003
- Ferric ammonium citrate -> 0.003
- EDTA -> 0.0005
- Na2C03 -> 0.02
- Trace metal mix -> 1ml (*)
- Sea water -> 750 ml
- Deionized water -> 250 ml
Gojišče ASN-I11 [g/liter gojišča]
- NaCl -> 25.0
- MgCI2.6H20 -> 2.0
- KC1 -> 0.5
- NaNO3 -> 0.75
- K2HPO4.3H2O -> 0.02
- MgS04.7H2O -> 3.5
- CaC12.2H20 -> 0.5
- Citric acid -> 0.003
- Ferric ammonium citrate -> 0.003
- EDTA -> 0.0005
- Na2C03 -> 0.02
- Trace metal mix -> 1 ml (*)
- Deionized water -> 1000 ml
(*) Trace metal mix: [g/liter]
- H3BO3 -> 2.86
- MnCI2.4H20 -> 1.81
- ZnSO4.7H2O -> 0.222
- Na2MoO4.2H20 -> 0.390
- CuSO4.5H20 -> 0.079
- Co(N03)2.6H20 -> 0.00494
